• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

SF3B5 Polyclonal antibody

SF3B5 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 15525-1-AP

Print datasheet

Synonyms

SF3b10, SF3B5, Splicing factor 3B subunit 5

验证数据展示

  • WB analysis of SH-SY5Y using 15525-1-AP

    WB analysis of SH-SY5Y using 15525-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15525-1-AP (SF3B5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15525-1-AP

    IF Staining of HeLa using 15525-1-AP

    Immunofluorescent analysis of Hela cells, using SF3B5 antibody 15525-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human ovary tumor using 15525-1-AP

    IHC staining of human ovary tumor using 15525-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15525-1-AP (SF3B5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF3B5 antibody included
  • IHC staining of human ovary tumor using 15525-1-AP

    IHC staining of human ovary tumor using 15525-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15525-1-AP (SF3B5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF3B5 antibody included

经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, mouse eye tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy SF3B5 Ready-To-Use IHC Kit. SF3B5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15525-1-AP targets SF3B5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen SF3B5 fusion protein Ag7205 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name splicing factor 3b, subunit 5, 10kDa
Synonyms SF3b10, SF3B5, Splicing factor 3B subunit 5
Calculated Molecular Weight 10 kDa
Observed Molecular Weight 10-12 kDa
GenBank Accession NumberBC000198
Gene Symbol SF3B5
Gene ID (NCBI) 83443
RRIDAB_10596628
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BWJ5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SF3B5 antibody 15525-1-APDownload protocol
IHC protocol for SF3B5 antibody 15525-1-APDownload protocol
IF protocol for SF3B5 antibody 15525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols