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验证数据展示

  • WB analysis of SH-SY5Y using 15525-1-AP

    WB analysis of SH-SY5Y using 15525-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15525-1-AP (SF3B5 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 15525-1-AP

    IF Staining of HeLa using 15525-1-AP

    Immunofluorescent analysis of Hela cells, using SF3B5 antibody 15525-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • IHC staining of human ovary tumor using 15525-1-AP

    IHC staining of human ovary tumor using 15525-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15525-1-AP (SF3B5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF3B5 antibody included
  • IHC staining of human ovary tumor using 15525-1-AP

    IHC staining of human ovary tumor using 15525-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 15525-1-AP (SF3B5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with SF3B5 antibody included

SF3B5 Polyclonal antibody

SF3B5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 15525-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

Splicing factor 3B subunit 5, SF3B10, Pre-mRNA-splicing factor SF3b 10 kDa subunit

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inSH-SY5Y cells, HeLa cells, mouse eye tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells
Planning an IHC experiment? We recommend our IHCeasy SF3B5 Ready-To-Use IHC Kit. SF3B5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15525-1-AP targets SF3B5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 SF3B5 fusion protein Ag7205 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 splicing factor 3b, subunit 5, 10kDa
别名 Splicing factor 3B subunit 5, SF3B10, Pre-mRNA-splicing factor SF3b 10 kDa subunit
计算分子量 10 kDa
观测分子量 10-12 kDa
GenBank蛋白编号BC000198
基因名称 SF3B5
Gene ID (NCBI) 83443
RRIDAB_10596628
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BWJ5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for SF3B5 antibody 15525-1-APDownload protocol
IHC protocol for SF3B5 antibody 15525-1-APDownload protocol
IF protocol for SF3B5 antibody 15525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols