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验证数据展示

  • IP experiment of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

    IP experiment of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

    IP result of anti-SERP1 (IP:17807-1-AP, 4ug; Detection:17807-1-AP 1:500) with mouse brain tissue lysate 3160 ug. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

    WB analysis of mouse brain using 17807-1-AP (same clone as 17807-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17807-1-AP (SERP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

    IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

    IHC staining of human endometrial cancer using 17807-1-AP (same clone as 17807-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer using 17807-1-AP (SERP1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 17807-1-AP (same clone as 17807-1-PBS)

    IF Staining of HeLa using 17807-1-AP (same clone as 17807-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using SERP1 antibody (17807-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 17807-1-PBS in a different storage buffer formulation.

SERP1 Polyclonal antibody, PBS Only

SERP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 17807-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

RAMP4, Ribosome-attached membrane protein 4, Stress-associated endoplasmic reticulum protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

17807-1-PBS targets SERP1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12087

Product name: Recombinant human SERP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-66 aa of BC108314

Sequence: MVAKQRIRMANEKHSKNITQRGNVAKTSRNAPEEKASVGPWLLALFIFVVCGSAIFQIIQSIRMGM

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 stress-associated endoplasmic reticulum protein 1
别名 RAMP4, Ribosome-attached membrane protein 4, Stress-associated endoplasmic reticulum protein 1
计算分子量 66 aa, 7 kDa
观测分子量 10 kDa
GenBank蛋白编号BC108314
基因名称 SERP1
Gene ID (NCBI) 27230
RRIDAB_10597394
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y6X1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Stress-associated endoplasmic reticulum (ER) protein 1 (SERP1), also known as ribosome- associated membrane protein 4 (RAMP4), is a Sec61-associated polypeptide that is induced by ER stress [PMID:16705175]. SERP1 interacts with target proteins during their translocation into the lumen of the endoplasmic reticulum. It controls glycosylation of major histocompatibility complex class II-associated invariant chains by a translocational pausing mechanism, and its overexpression stabilizes newly synthesized membrane proteins under ER stress by associating with the Sec61 complex [PMID:10601334]. It is suggested SERP1 is involved in the biosynthesis/processing of secretory proteins

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