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验证数据展示

  • WB analysis using 86646-1-RR (same clone as 86646-1-PBS)

    WB analysis using 86646-1-RR (same clone as 86646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86646-1-RR (SCRN1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86646-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 86646-1-RR (same clone as 86646-1-PBS)

    IF Staining of A549 using 86646-1-RR (same clone as 86646-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed A549 cells using SCRN1 antibody (86646-1-RR, Clone: 251548C7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86646-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86646-1-RR

    Affinity and Kinetic Characterization of 86646-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86646-1-RR against Human SCRN1 were performed. The affinity constant is 5.99 nM.

SCRN1 Recombinant monoclonal antibody, PBS Only

SCRN1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 86646-1-PBS

产品说明书

CloneNo. 251548C7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA0193, secernin 1, Secernin-1, SES1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

86646-1-PBS targets SCRN1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag5413

Product name: Recombinant human SCRN1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-414 aa of BC040492

Sequence: MAAAPPSYCFVAFPPRAKDGLVVFGKNSARPRDEVQEVVYFSAADHEPESKVECTYISIDQVPRTYAIMISRPAWLWGAEMGANEHGVCIANEAINTREPAAEIEALLGMDLVRLGLERGETAKEALDVIVSLLEEHGQGGNYFEDANSCHSFQSAYLIVDRDEAWVLETIGKYWAAEKVTEGVRCICSQLSLTTKMDAEHPELRSYAQSQGWWTGEGEFNFSEVFSPVEDHLDCGAGKDSLEKQEESITVQTMMNTLRDKASGVCIDSEFFLTTASGVSVLPQNRSSPCIHYFTGTHDPSRSIFKPFIFVDDVKLVPKTQSPCFGDDDPAKKEPRFQEKPDRRHELYKAHEWARAIIESDQEQGRKLRSTMLELEKQGLEAMEEILTSSEPLDPAEVGDLFYDCVDTEIKFFK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 secernin 1
别名 KIAA0193, secernin 1, Secernin-1, SES1
计算分子量 46 kDa
观测分子量 50 kDa
GenBank蛋白编号BC040492
基因名称 SCRN1
Gene ID (NCBI) 9805
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ12765
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

SCRN genes encode protein members of the secernin family including Secernin 1, Secernin 2 and Secernin 3. Function of Secernin 2 and Secernin 3 are so far not well understood. Secernin 1 is a novel 50-kDa cytosolic protein that appears to be involved in the regulation of exocytosis from peritoneal mast cells. Secernin-1 a novel tumor-associated antigen (TAA) and may be a universal marker of different cancer types including gastric cancer, which has been validated by several researches. Measurement of SCRN1 can be used in the early detection of cancer or in the surveillance of patients who undergo surgery.

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