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验证数据展示

  • WB analysis using 28174-1-AP (same clone as 28174-1-PBS)

    WB analysis using 28174-1-AP (same clone as 28174-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28174-1-AP (SCARF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28174-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 28174-1-AP (same clone as 28174-1-PBS)

    IF Staining of HepG2 using 28174-1-AP (same clone as 28174-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SCARF1 antibody (28174-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28174-1-PBS in a different storage buffer formulation.

SCARF1 Polyclonal antibody, PBS Only

SCARF1 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28174-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Acetyl LDL receptor, KIAA0149, Scavenger receptor class F member 1, Scavenger receptor expressed by endothelial cells 1, SREC

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

28174-1-PBS targets SCARF1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag28079

Product name: Recombinant human SCARF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 695-830 aa of BC039735

Sequence: AGKPRGSEGPVRSVFRHFGSFQKGQAEAKVKRAIPKPPRQALNRKKGSPGLASGSVGQSPNSAPKAGLPGATGPMAVRPEEAVRGLGAGTESSRRAQEPVSGCGSPEQDPQKQAEEERQEEPEYENVVPISRPPEP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 scavenger receptor class F, member 1
别名 Acetyl LDL receptor, KIAA0149, Scavenger receptor class F member 1, Scavenger receptor expressed by endothelial cells 1, SREC
计算分子量 830 aa, 87 kDa
观测分子量90 kDa, 180 kDa
GenBank蛋白编号BC039735
基因名称 SCARF1
Gene ID (NCBI) 8578
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14162
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Scavenger receptor class F member 1 (SCARF1), also known as scavenger receptor expressed by endothelial cells (SREC)-I, belongs to the scavenger receptor (SR) superfamily. SCARF1 has been shown to bind modified low density lipoproteins (LDLs), including acetylated (Ac-) and oxidized (ox-) LDLs (PMID: 29725698; PMID: 15145948). In addition to binding and internalizing a diverse range of endogenous proteins, SCARF1 also binds a wide array of viral, fungal, and bacterial antigens (PMID: 29242513). SCARF1 has a calculated molecular weight of 87 kDa, with a molecular weight of 140-160 kDa due to N-glycosylation (PMID: 22279061; PMID: 15145948). SCARF1 can also exist as a dimer (~ 180 kDa) and a truncated soluble form (~ 60 kDa) (PMID: 29242513; PMID: 29725698).

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