Rhodamine 110

Rhodamine 110 (RH110) 是灵敏的选择性底物,激发波长为 498 nm,发射波长为 521 nm,可用于测定溶液或活细胞内的蛋白酶。

CAS号

13558-31-1

分子式

C20H15ClN2O3

主要靶点

Others

仅限科研使用

Cat No : CM02247

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Synonyms

RH110|Rhodamine 110 chloride|罗丹明110



产品信息

Rhodamine 110 is a xanthene dye similar to fluorescein and eosin.

CAS号 13558-31-1
分子式 C20H15ClN2O3
主要靶点 Others
主要通路 其他
分子量 366.8
纯度 98.13%, 此纯度可做参考,具体纯度与批次有关系,可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year
别名 RH110|Rhodamine 110 chloride|罗丹明110

体内活性

Rhodamine 110 is less toxic than the parent molecule based on the intravenous LD50 acute toxicity values of 89.5 mg/kg and 140.0 mg/kg for Rhodamine B and Rhodamine 110, respectively. Testis weight increased in male rats dosed with Rhodamine 110. Both molecules induce liver and kidney enlargement after ingestion, and male rats show more significant increases than female rats after Rhodamine 110 exposure. The pharmacokinetics of Rhodamine 110 are assessed following oral administration at two dosages (3 and 10 mg/kg) and intravenous administration at one dosage (3 mg/kg). Pharmacokinetic parameters are calculated using an extravascular input and IV-bolus input, noncompartmental model analysis conducted with WinNonlin Standard Edition. The pharmacokinetic data demonstrate that the AUC is proportional to the administered oral dose of Rhodamine 110 (3 mg/kg and 10 mg/kg). Furthermore, the clearance (Cl) of the two orally administered doses is 7.94 and 8.61 mL/min/kg, respectively[1]. The pharmacokinetic parameters of Rhodamine 110 indicates that the maximum plasma concentrations (Cmax) of the two oral dosages are 283.4 and 657.0 ng/mL, which are reached at 140 and 210 min, respectively. This indicates that Rhodamine 110 absorption is not rapid after ingestion, as it took over 2 h to be absorbed from the intestines into the blood. The areas under the concentration–time curves (AUCs) for the two dosages are 138.1±20.3 and 444.0±170.8 h ng/mL.

体外活性

Rhodamine 110 accumulates in mitochondria in a cationic form, which alters the pH in this cellular compartment. No cytotoxicity to human lymphoblastoid cells is observed below 10 μM, but Rhodamine 110 causes Friend leukemia cells to die at a concentrations above 100 μM[1].

溶解度

DMSO:25 mg/mL (68.16 mM),Sonification/heating is recommended.

参考文献

1.Jiang SH, et al. Pharmacokinetics of Rhodamine 110 and Its Organ Distribution in Rats. J Agric Food Chem. 2017 Sep 6;65(35):7797-7804.

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The dilution calculator equation

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