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Retinoic acid

CAS号

302-79-4

分子式

C20H28O2

主要靶点

Endogenous Metabolite|Autophagy|Retinoid Receptor|PPAR

仅限科研使用

Cat No : CM01288

Print datasheet

Synonyms

Inhibitor|PPAR|Peroxisome proliferator-activated receptors|Retinoic acid|Retinoic acid receptors|Retinoid Receptor|RAR|RAR/RXR|RetinoidReceptor|Retinoid X receptors|维生素A酸|Vitamin A acid|Tretinoin|ATRA|Autophagy|all-trans-Retinoic acid|Endogenous Metabolite|inhibit|EndogenousMetabolite

验证数据展示

  • CAS No.: 302-79-4

    Retinoic acid
    CAS#: 302-79-4

产品信息

CAS号 302-79-4
分子式 C20H28O2
主要靶点 Endogenous Metabolite|Autophagy|Retinoid Receptor|PPAR
主要通路 代谢|DNA 损伤和修复|代谢|代谢|自噬
分子量 300.44
纯度 99.6%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 keep away from direct sunlight,store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 Inhibitor|PPAR|Peroxisome proliferator-activated receptors|Retinoic acid|Retinoic acid receptors|Retinoid Receptor|RAR|RAR/RXR|RetinoidReceptor|Retinoid X receptors|维生素A酸|Vitamin A acid|Tretinoin|ATRA|Autophagy|all-trans-Retinoic acid|Endogenous Metabolite|inhibit|EndogenousMetabolite

靶点活性

RARα:14 nM|RARβ:14 nM|RARγ:14 nM

体内活性

方法：为研究对小鼠齿筋膜兴奋性和抑制性神经传递的影响，将 Retinoic acid (10 mg/kg in Corn oil+5% DMSO) 单次腹腔注射给 C57BL/6J 小鼠。 结果：在给予 Retinoic acid 6 h 后，背侧海马中的自发兴奋性突触后电流频率和突触数量均显著增加。Retinoic acid 介导小鼠齿状颗粒细胞中突触足蛋白依赖性化生。[3] 方法：为检测对小鼠肝脏脂质代谢的影响，将 Retinoic acid (10-100 mg/kg) 皮下注射给 NMRI 小鼠，每天一次，持续四天。 结果：Retinoic acid 治疗可减轻体重和肥胖。Retinoic acid 在体内将肝脏脂质代谢转变为增加分解代谢和减少脂肪生成。[4]

体外活性

方法：人单核细胞 THP-1 用 Retinoic acid (10-100 nM) 处理 24 h，使用 Western Blot 方法检测靶点蛋白表达水平。 结果：Retinoic acid 处理的细胞中 RXRα 和 RARα 蛋白持续减少。[1] 方法：小鼠胚胎干细胞 mESC 用 Retinoic acid (1 μM) 处理 24 h，进行基因表达微阵列分析。 结果：Retinoic acid 处理后分化相关基因 Hoxb1、Hoxb2 和 Hoxb3 上调，而多能性相关基因 Oct4、Nanog、Klf4、Esrrb、Lefty1 和 Letfy2 下调。Retinoic acid 诱导ES细胞外胚层标志物表达。[2]

溶解度

H2O:< 1 mg/mL (insoluble or slightly soluble);Ethanol:6 mg/mL (19.97 mM);DMSO:45 mg/mL (149.78 mM);10% DMSO+40% PEG300+5% Tween 80+45% Saline:5.6 mg/mL (18.64 mM)

细胞实验

Retinoic acid is dissolved in DMSO and stored, and then diluted with appropriate medium before use[3]. P19 cell are induced to undergo neuronal differentiation according to established procedures. Briefly, cells are cultured on 1% agarose-coated 10 cm dishes at 3×10 5 cells/mL in α-minimal essential medium supplemented with 10% FBS. Differentiation is induced by addition of Retinoic acid (1 μM) and medium containing Retinoic acid replaced 2 days later. On day 4, cell aggregates are collected by centrifugation, separated to single cells by trypsin/EDTA treatment, replated onto poly-L-lysine-coated plates, and cultured in α-minimal essential medium supplemented with 10% FBS. On day 6, medium is replaced with neurobasal medium containing B27 supplement and 2 mM GlutaMAX. Medium is replaced every 2 days for an additional week[3].

参考文献

1.Chen Q, et al. Retinoic acid regulates cell cycle progression and cell differentiation in human monocytic THP-1 cells. Exp Cell Res. 2004 Jul 1;297(1):68-81.
2.Qiu Y, Sun Y, Xu D, et al. Screening of FDA-approved drugs identifies sutent as a modulator of UCP1 expression in brown adipose tissue[J]. EBioMedicine. 2018, 37: 344-355.
3.Zhang J, et al. Retinoic Acid Induces Embryonic Stem Cell Differentiation by Altering Both Encoding RNA and microRNA Expression. PLoS One. 2015 Jul 10;10(7):e0132566.
4.Lenz M, et al. All-trans retinoic acid induces synaptopodin-dependent metaplasticity in mouse dentate granule cells. Elife. 2021 Nov 1;10:e71983.
5.Amengual J, et al. Retinoic acid treatment enhances lipid oxidation and inhibits lipid biosynthesis capacities in the liver of mice. Cell Physiol Biochem. 2010;25(6):657-66.
6.Muehlberger T, et al. J Am Acad Dermatol, 2005, 52(4), 583-588.
7.Wu L, et al. Retinoid X Receptor Agonists Upregulate Genes Responsible for the Biosynthesis of All-Trans-Retinoic Acid in Human Epidermis. PLoS One. 2016 Apr 14;11(4):e0153556.
8.Wan X Q, Cai J Y, Zhu Y, et al. SENP1 has an important role in lung development and influences the differentiation of alveolar type 2 cells[J]. International journal of molecular medicine. 2019 Jan;43(1):371-381.
9.Wei Z, Li T, Sun Y, et al. Daturataturin A, a withanolide in Datura metel L., induces HaCaT autophagy through the PI3K‐Akt‐mTOR signaling pathway[J] . Phytotherapy Research. 2021
10.Yanxing Wang, Xiaodong Dou, Lan Jiang, Hongwei Jin, Lihe Zhang, Liangren Zhang, and Zhenming Liu. Discovery of novel glycogen synthase kinase-3α inhibitors: Structurebased virtual screening, preliminary SAR and biological evaluation for treatment of acute myeloid leukemia [J]. European Journal of Medicinal Chemistry. 2019 Jun 1;171:221-234.

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