Recombinant Rat t-Plasminogen activator/tPA protein (rFc Tag)

种属

Rat

纯度

>90 %, SDS-PAGE

标签

rFc Tag

生物活性

未测试

Cat no : Eg3260

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Synonyms

EC:3.4.21.68, Plat, Tissue-type plasminogen activator, Tissue-type plasminogen activator chain A, Tissue-type plasminogen activator chain B



产品信息

纯度 >90 %, SDS-PAGE
内毒素 <0.1 EU/μg protein, LAL method
生物活性
Not tested
来源 HEK293-derived Rat t-Plasminogen activator protein Ser33-Gln559 (Accession# P19637) with a rabbit IgG Fc tag at the C-terminus.
基因ID 25692
蛋白编号 P19637
预测分子量 85.6 kDa
SDS-PAGE 28-32 kDa, 36-38 kDa and 60-70 kDa, reducing (R) conditions
组分 Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4. Normally 5% trehalose and 5% mannitol are added as protectants before lyophilization.
复溶 Briefly centrifuge the tube before opening. Reconstitute at 0.1-0.5 mg/mL in sterile water.
储存条件
It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
  • Until expiry date, -20℃ to -80℃ as lyophilized proteins.
  • 3 months, -20℃ to -80℃ under sterile conditions after reconstitution.
运输条件 The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.

背景信息

Tissue‐type plasminogen activator (tPA) is a secreted serine protease of the trypsin family that catalyzes the critical step in fibrinolysis. It activates the circulating enzyme plasminogen, which can breakdown insoluble fibrin in the thrombus into soluble fibrin degradation products (FDPs). As thrombolytic agents, plasminogen activators are widely used to clear circulatory blockages caused by fibrin clots or thrombus. Recombinant tissue plasminogen activator (r‐tPA) has been approved by the US Food and Drug Administration as a clinical thrombolytic drug.

参考文献:

1. Collen D. et al. (1991). Blood. 78:3114‐3124. 2. Henderson SJ. et al. (2018). J Thromb Haemost. 16:1932‐1940. 3. Prabhakaran S. et al. (2015). JAMA. 313:1451‐1462.

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