Speedy™ Rat Lipocalin-2/NGAL One-Step ELISA Kit
Sensitivity
0.1 pg/mL
Range
15.6-1000 pg/mL
Reactivity
Rat
Cat no : SE70008
Synonyms
Lcn2, Lipocalin2/NGAL, Alpha-2-microglobulin-related protein, Alpha-2U globulin-related protein, lipocalin 2
Validation Data Gallery
Product Information
| Product name | Speedy™ Rat Lipocalin-2/NGAL One-Step ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Serum, Plasma, Urine, Tissue lysate |
| Assay type | Sandwich |
| Sensitivity | 0.1 pg/mL |
| Range | 15.6-1000 pg/mL |
| Reactivity | Rat |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 170496 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Rat serum | 96% | 91%-101% |
| Urine | 92% | 90%-96% |
| Tissue lysate | 91% | 76%-122% |
IntraAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 8 | 503.0 | 7.9 | 1.6 |
| 2 | 8 | 128.9 | 2.1 | 1.6 |
| 3 | 8 | 68.5 | 1.8 | 2.6 |
InterAssay
| Sample | n | mean ( pg/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 16 | 513.5 | 13.4 | 2.6 |
| 2 | 16 | 130.7 | 2.8 | 2.1 |
| 3 | 16 | 70.5 | 2.7 | 3.8 |
Background Information
Neutrophil gelatinase-associated lipocalin (NGAL) also known as Lipocalin-2 (LCN2), is a secreted glycoprotein that belongs to the lipocalin protein family. Several roles of NGAL were identified, including iron trafficking, chemotactic, and promoting cell differentiation and proliferation. NGAL was originally found in neutrophils and expressed in tissues containing renal, uterus, liver, spleen, cardiomyocytes, neurons, and immune cells. NGAL is a significant biomarker of cardiovascular diseases, ischemic stroke, acute renal injury, inflammation, and metabolic diseases.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | Lcn2, Lipocalin2/NGAL, Alpha-2-microglobulin-related protein, Alpha-2U globulin-related protein, lipocalin 2 |
