- Featured Product
- KD/KO Validated
RUNX2 Polyclonal antibody
RUNX2 Polyclonal Antibody for FC, IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse and More (1)
Applications
FC, IF, IP, WB, ELISA and More (2)
Conjugate
Unconjugated
110
验证数据展示
经过测试的应用
Positive WB detected in | ROS1728 cells, MCF-7 cells |
Positive IP detected in | ROS1728 cells |
Positive IF detected in | mouse brain tissue |
Positive FC detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20700-1-AP targets RUNX2 in WB, IP, IHC, IF, FC, chIP, ELISA applications and shows reactivity with human, rat, mouse samples.
Tested Applications | FC, IF, IP, WB, ELISA |
Cited Applications | chIP, IF, IHC, WB |
Tested Reactivity | human, rat, mouse |
Cited Reactivity | human, mouse, rat, chicken |
Immunogen | Peptide 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | runt-related transcription factor 2 |
Synonyms | AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2 |
Calculated Molecular Weight | 57 kDa |
Observed Molecular Weight | 57-60 kDa |
GenBank Accession Number | NM_004348 |
Gene Symbol | RUNX2 |
Gene ID (NCBI) | 860 |
RRID | AB_2722783 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q13950 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for RUNX2 antibody 20700-1-AP | Download protocol |
IF protocol for RUNX2 antibody 20700-1-AP | Download protocol |
IP protocol for RUNX2 antibody 20700-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Mater Today Bio Sustained release of naringin from silk-fibroin-nanohydroxyapatite scaffold for the enhancement of bone regeneration. | ||
ACS Appl Mater Interfaces Synergistic Effect of Magneto-Mechanical Bioengineered Stem Cells and Magnetic Field to Alleviate Osteoporosis | ||
Clin Immunol STING contributes to trauma-induced heterotopic ossification through NLRP3-dependent macrophage pyroptosis | ||
Br J Pharmacol Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease. | ||
Oxid Med Cell Longev Hsa_circRNA_0008028 Deficiency Ameliorates High Glucose-Induced Proliferation, Calcification, and Autophagy of Vascular Smooth Muscle Cells via miR-182-5p/TRIB3 Axis | ||
Int J Nanomedicine The Role of Tantalum Nanoparticles in Bone Regeneration Involves the BMP2/Smad4/Runx2 Signaling Pathway. |