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RUNX2 Polyclonal antibody

RUNX2 Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat, mouse and More (1)

Applications

IF, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 20700-1-AP

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Synonyms

AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2



经过测试的应用

Positive WB detected inROS1728 cells, MCF-7 cells
Positive IF detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

产品信息

20700-1-AP targets RUNX2 in WB, IHC, IF, chIP, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications IF, WB, ELISA
Cited Applications chIP, IF, IHC, WB
Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse, rat, chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name runt-related transcription factor 2
Synonyms AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2
Calculated molecular weight 57 kDa
Observed molecular weight 57-60 kDa
GenBank accession numberNM_004348
Gene symbol RUNX2
UniprotQ13950
Gene ID (NCBI) 860
RRIDAB_2722783
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RUNX2, also named as AML3, CBFA1, OSF2 and PEBP2A, is a Transcription factor which involved in osteoblastic differentiation and skeletal morphogenesis. It is essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters By similarity. RUNX2 inhibits MYST4-dependent transcriptional activation. The antibody is specific to RUNX2.

实验方案

Product Specific Protocols
WB protocol for RUNX2 antibody 20700-1-APDownload protocol
IF protocol for RUNX2 antibody 20700-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Br J Pharmacol

Hydrogen sulfide inhibits calcification of heart valves; implications for calcific aortic valve disease.

Authors - Katalin Éva Sikura
humanWB

BMC Med

Loss of thymidine phosphorylase activity disrupts adipocyte differentiation and induces insulin-resistant lipoatrophic diabetes.

Authors - Jérémie Gautheron
humanWB

Arterioscler Thromb Vasc Biol

Hypoxia Triggers Osteochondrogenic Differentiation of Vascular Smooth Muscle Cells in an HIF-1 (Hypoxia-Inducible Factor 1)-Dependent and Reactive Oxygen Species-Dependent Manner.

Authors - Enikő Balogh
ratWB

Mater Sci Eng C Mater Biol Appl

A polydopamine-assisted strontium-substituted apatite coating for titanium promotes osteogenesis and angiogenesis via FAK/MAPK and PI3K/AKT signaling pathways.

Authors - Yiting Sun
mouseWB

J Cell Physiol

miR-142a-5p promoted osteoblast differentiation via targeting nuclear factor IA.

Authors - Hairui Yuan
humanWB,IF

Front Cell Dev Biol

Long Non-coding RNAs LOC100126784 and POM121L9P Derived From Bone Marrow Mesenchymal Stem Cells Enhance Osteogenic Differentiation via the miR-503-5p/SORBS1 Axis.

Authors - Yiyang Xu