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验证数据展示

  • WB analysis using 15315-1-AP (same clone as 15315-1-PBS)

    WB analysis using 15315-1-AP (same clone as 15315-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15315-1-AP (RRAGD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15315-1-PBS in a different storage buffer formulation.

  • IP experiment of NIH/3T3 using 15315-1-AP (same clone as 15315-1-PBS)

    IP experiment of NIH/3T3 using 15315-1-AP (same clone as 15315-1-PBS)

    IP result of anti-RRAGD (IP:15315-1-AP, 4ug; Detection:15315-1-AP 1:500) with NIH/3T3 cells lysate 1120 ug. This data was developed using the same antibody clone with 15315-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15315-1-AP (same clone as 15315-1-PBS)

    IF Staining of HepG2 using 15315-1-AP (same clone as 15315-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RRAGD antibody (15315-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 15315-1-PBS in a different storage buffer formulation.

RRAGD Polyclonal antibody, PBS Only

RRAGD Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 15315-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

EC:3.6.5.-, Rag D, RagD, Ras related GTP binding D, Ras-related GTP-binding protein D

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15315-1-PBS targets RRAGD in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7557

Product name: Recombinant human RRAGD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-249 aa of BC003088

Sequence: MEALARLHLTVTRAYKVNTDINFEVFIHKVDGLSDDHKIETQRDIHQRANDDLADAGLEKIHLSFYLTSIYDHSIFEAFSKVVQKLIPQLPTLENLLNIFISNSGIEKAFLFDVVSKIYIATDSTPVDMQTYELCCDMIDVVIDISCIYGLKEDGAGTPYDKESTAIIKLNNTTVLYLKEVTKFLALVCFVREESFERKGLIDYNFHCFRKAIHEVFEVRMKVVKSRKVQNRLQKKKRATPNGTPRVLL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras-related GTP binding D
别名 EC:3.6.5.-, Rag D, RagD, Ras related GTP binding D, Ras-related GTP-binding protein D
计算分子量 46 kDa
观测分子量46-50 kDa
GenBank蛋白编号BC003088
基因名称 RRAGD
Gene ID (NCBI) 58528
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQL2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ras-related GTP-binding protein D (RRAGD) is a monomeric GTP or GDP binding protein, which plays a critical role in mediating amino acid stimulated mTOR signaling pathway, a key pathway in determination of cell growth and proliferation rate. Recently, RRAGD has been reported involving the progression of ovarian cancer and colon cancer.

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