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验证数据展示

  • WB analysis using 15799-1-AP (same clone as 15799-1-PBS)

    WB analysis using 15799-1-AP (same clone as 15799-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15799-1-AP (RPL29 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 15799-1-AP (same clone as 15799-1-PBS)

    IHC staining of mouse brain using 15799-1-AP (same clone as 15799-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15799-1-AP (RPL29 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 15799-1-AP (same clone as 15799-1-PBS)

    IF Staining of HepG2 using 15799-1-AP (same clone as 15799-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL29 antibody (15799-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15799-1-PBS in a different storage buffer formulation.

RPL29 Polyclonal antibody, PBS Only

RPL29 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 15799-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15799-1-PBS targets RPL29 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8510

Product name: Recombinant human RPL29 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-159 aa of BC008926

Sequence: MAKSKNHTTHNQSRKWHRNGIKKPRSQRYESLKGVDPKFLRNMRFAKKHNKKGLKKMQANNAKAMSARAEAIKALVKPKEVKPKIPKGVSRKLDRLAYIAHPKLGKRARARIAKGLRLCRPKAKAKAKAKDQTKAQAAAPASVPAQAPKRTQAPTKASE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ribosomal protein L29
别名
计算分子量 159 aa, 18 kDa
观测分子量 20-25 kDa
GenBank蛋白编号BC008926
基因名称 RPL29
Gene ID (NCBI) 6159
RRIDAB_2878187
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP47914
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Ribosomal protein L29 (Rpl29) is a component of the large (60S) ribosomal subunit which is abundantly expressed in all cell types, plays a regulatory role in translation efficiency and is essential for protein synthesis (PMID: 17195189). In contrast to adult tissues, RPL29 displays a much more widespread pattern of expression throughout embryonic development, and high levels of RPL29 are found in all developing organs. Several reports showed that RPL29 expression is up-regulated in human carcinomas and constitutes a candidate marker for abnormal growth when overexpressed(PMID: 9788632, 10383165).The antibody can detect the band around 22kDa.

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