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验证数据展示

  • WB analysis using 23927-1-AP

    WB analysis using 23927-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23927-1-AP (RNF146 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary cancer using 23927-1-AP

    IHC staining of human ovary cancer using 23927-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 23927-1-AP (RNF146 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

RNF146 Polyclonal antibody

RNF146 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 23927-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Iduna, RING-type E3 ubiquitin transferase RNF146, RING finger protein 146, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF146

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

23927-1-AP targets RNF146 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 RNF146 fusion protein Ag20999 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 ring finger protein 146
别名 Iduna, RING-type E3 ubiquitin transferase RNF146, RING finger protein 146, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF146
计算分子量 359 aa, 39 kDa
观测分子量40-45 kDa
GenBank蛋白编号BC008235
基因名称 RNF146
Gene ID (NCBI) 81847
RRIDAB_3669427
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen Affinity purified
UNIPROT IDQ9NTX7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RNF146 antibody 23927-1-APDownload protocol
IHC protocol for RNF146 antibody 23927-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols