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验证数据展示

  • WB analysis using 31223-1-AP

    WB analysis using 31223-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31223-1-AP (RIN2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

RIN2 Polyclonal antibody

RIN2 Polyclonal Antibody for WB, ELISA
Cat No. 31223-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

Ras and Rab interactor 2, Ras inhibitor JC265, RASSF4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHepG2 cells, L02 cells, LNCaP cells, PC-3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31223-1-AP targets RIN2 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 RIN2 fusion protein Ag35011 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Ras and Rab interactor 2
别名 Ras and Rab interactor 2, Ras inhibitor JC265, RASSF4
计算分子量100 kDa
观测分子量100-120 kDa
GenBank蛋白编号NM_018993
基因名称 RIN2
Gene ID (NCBI) 54453
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8WYP3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

RIN2 (Ras and Rab interactor 2), also known as RASSF4. RIN2 is mainly located in Golgi apparatus and participates in the process of material transport and secretion in cells. It is also distributed in cytoplasmic matrix, which may be related to signal transduction in cells. It is highly expressed in the brain, and also in the cerebellum, spinal cord and other nervous system tissues. RIN2 belongs to the family of Ras and Rab exchange factors and has a unique VPS9 domain. It can be used as a nucleotide exchange factor to activate the downstream small GTP enzymes Rab5 and R-Ras, thus starting intracellular signal transduction and regulating cytoskeleton recombination, synapse secretion and cell adhesion. The molecular weight of RIN2 is 100 kDa.

实验方案

Product Specific Protocols
WB protocol for RIN2 antibody 31223-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols