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验证数据展示

  • WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

    WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

  • WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

    WB analysis using 12547-1-AP (same clone as 12547-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 12547-1-AP (RGS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 12547-1-AP (same clone as 12547-1-PBS)

    WB analysis of HepG2 using 12547-1-AP (same clone as 12547-1-PBS)

    WB result of RGS1 antibody (12547-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-RGS1 transfected HepG2 cells. This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 12547-1-AP (same clone as 12547-1-PBS)

    IHC staining of human stomach cancer using 12547-1-AP (same clone as 12547-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12547-1-AP (RGS1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 12547-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

RGS1 Polyclonal antibody, PBS Only

RGS1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12547-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

IER1, Early response protein 1R20, BL34, B-cell activation protein BL34, B cell activation protein BL34

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12547-1-PBS targets RGS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3315

Product name: Recombinant human RGS1 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-196 aa of BC015510

Sequence: MPGMFFSANPKELKGTTHSLLDDKMQKRRPKTFGMDMKAYLRSMIPHLESGMKSSKSKDVLSAAEVMQWSQSLEKLLANQTGQNVFGSFLKSEFSEENIEFWLACEDYKKTESDLLPCKAEEIYKAFVHSDAAKQINIDFRTRESTAKKIKAPTPTCFDEAQKVIYTLMEKDSYPRFLKSDIYLNLLNDLQANSLK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 regulator of G-protein signaling 1
别名 IER1, Early response protein 1R20, BL34, B-cell activation protein BL34, B cell activation protein BL34
计算分子量 196 aa, 22 kDa
观测分子量 30 kDa
GenBank蛋白编号BC015510
基因名称 RGS1
Gene ID (NCBI) 5996
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ08116
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

RGS1 (Regulator of G-protein signaling 1) is also named as 1R20, BL34 and IER1. RGS1 encodes a member of the regulator of G-protein signaling family, which can act as a GTPase-activating protein (GAP), increasing the rate of conversion of the GTP to GDP, driving G-protein into its inactive GDP-bound form, hence attenuating or turning off G-protein-coupled receptor signaling (PMID: 8774882). RGS1 is highly expressed in immune cells including T cells, B cells, natural killer (NK) cells, dendritic cells and monocytes, suggesting a role for RGS1 in immune cell regulation (PMID: 34956194). RGS1 was highly expressed in tumor tissues and correlated with shorter overall survival, which also appeared in several previous studies, including multiple myeloma, melanoma, nonsmall cell lung cancer, gastric cancer , diffuse large B-cell lymphoma (PMID: 34956194). RGS1 protein, located at the cytoplasm and membrane, is enriched in tumor tissues compared with normal tissues. RGS1 was identified as a HIF-dependent hypoxia target that dampens cell migration and signal transduction (PMID: 24686421).

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