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验证数据展示

  • WB analysis of Raji using 22242-1-AP (same clone as 22242-1-PBS)

    WB analysis of Raji using 22242-1-AP (same clone as 22242-1-PBS)

    Raji cells were subjected to SDS PAGE followed by western blot with 22242-1-AP (REST antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 22242-1-AP (same clone as 22242-1-PBS)

    WB analysis of HEK-293 using 22242-1-AP (same clone as 22242-1-PBS)

    WB result of REST antibody (22242-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-REST transfected HEK-293 cells. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 22242-1-AP (same clone as 22242-1-PBS)

    IHC staining of human breast cancer using 22242-1-AP (same clone as 22242-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22242-1-AP (REST antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with REST antibody included
  • IP experiment of HeLa using 22242-1-AP (same clone as 22242-1-PBS)

    IP experiment of HeLa using 22242-1-AP (same clone as 22242-1-PBS)

    IP result of anti-REST (IP:22242-1-AP, 4ug; Detection:22242-1-AP 1:15000) with HeLa cells lysate 1750 ug. This data was developed using the same antibody clone with 22242-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

REST Polyclonal antibody, PBS Only

REST Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 22242-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

NRSF, X2 box repressor, XBR

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy REST Ready-To-Use IHC Kit. REST primary antibody included.

产品信息

22242-1-PBS targets REST in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag17707

Product name: Recombinant human REST protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 748-1097 aa of BC132859

Sequence: ELSPPMEVVQKEPVKIELSPPIEVVQKEPVQMELSPPMGVVQKEPAQREPPPPREPPLHMEPISKKPPLRKDKKEKSNMQSERARKEQVLIEVGLVPVKDSWLLKESVSTEDLSPPSPPLPKENLREEASGDQKLLNTGEGNKEAPLQKVGAEEADESLPGLAANINESTHISSSGQNLNTPEGETLNGKHQTDSIVCEMKMDTDQNTRENLTGINSTVEEPVSPMLPPSAVEEREAVSKTALASPPATMAANESQEIDEDEGIHSHEGSDLSDNMSEGSDDSGLHGARPVPQESSRKNAKEALAVKAAKGDFVCIFCDRSFRKGKDYSKHLNRHLVNVYYLEEAAQGQE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RE1-silencing transcription factor
别名 NRSF, X2 box repressor, XBR
计算分子量 1097 aa, 122 kDa
观测分子量 200 kDa
GenBank蛋白编号BC132859
基因名称 REST
Gene ID (NCBI) 5978
RRIDAB_2879044
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13127
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

REST also named as NRSF or X2 box repressor is a 1097 amino acid protein, which contains 9 C2H2-type zinc fingers. REST as transcriptional repressor binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Repressor element 1 (RE1) silencing transcription factor (REST), is widely expressed during embryogenesis, and plays a strategic role in terminal neuronal differentiation. The molecular weight of REST is 121 kDa, but REST was detected 200kDa by western blot in our result, which is similar to the one article (PMID: 16764822).

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