- Featured Product
- KD/KO Validated
RENALASE Polyclonal antibody
RENALASE Polyclonal Antibody for IF/ICC,IF-P, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, rat, mouse
Applications
IF/ICC,IF-P, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Caco-2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse kidney tissue, HEK-293 cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
15003-1-AP targets RENALASE in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| Tested Applications | IF/ICC,IF-P, IHC, IP, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human, rat, mouse |
| Cited Reactivity | human, mouse, rat |
| Immunogen | RENALASE fusion protein Ag13061 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | chromosome 10 open reading frame 59 |
| Synonyms | C10orf59, MAO C, Monoamine oxidase C, RENALASE, RNLS |
| Calculated Molecular Weight | 38 kDa |
| Observed Molecular Weight | 35 kDa |
| GenBank Accession Number | BC005364 |
| Gene Symbol | C10orf59 |
| Gene ID (NCBI) | 55328 |
| RRID | AB_1557298 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VYX0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RNLS, also named as Renalase, C10orf59 and MAO-C, belongs to the renalase family. It is probable FAD-dependent amine oxidase secreted by the kidney, which circulates in blood and modulates cardiac function and systemic blood pressure. RNLS degrades catecholamines such as dopamine, norepinephrine and epinephrine in vitro. It lowers blood pressure in vivo by decreasing cardiac contractility and heart rate and preventing a compensatory increase in peripheral vascular tone, suggesting a causal link to the increased plasma catecholamine and heightened cardiovascular risk. High concentrations of catecholamines activate plasma renalase and promotes its secretion and synthesis. RNLS has physiologically relevant catecholamine-oxidizing activity. (PMID:15841207 ) This antibody is specific to RNLS.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RENALASE antibody 15003-1-AP | Download protocol |
| IHC protocol for RENALASE antibody 15003-1-AP | Download protocol |
| IF protocol for RENALASE antibody 15003-1-AP | Download protocol |
| IP protocol for RENALASE antibody 15003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Clin Invest Renalase is a novel, soluble monoamine oxidase that regulates cardiac function and blood pressure. | ||
Front Cardiovasc Med Aerobic Exercise Training Improves Renal Injury in Spontaneously Hypertensive Rats by Increasing Renalase Expression in Medulla. | ||
Life Sci Renalase is localized to the small intestine crypt and expressed upon the activation of NF-κB p65 in mice model of fasting-induced oxidative stress. | ||
Exp Biol Med (Maywood) Plasma and urine renalase levels and activity during the recovery of renal function in kidney transplant recipients. |
