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RBPJ antibody (mAb) (Clone 7A11)

Host / Isotype

Rat / IgG2b

Reactivity

Human

Applications

WB

CloneNo.

7A11

Cat No : 61073,61074 61073

Synonyms

CBF-1, cbf1, RBPJk, RBPJ1, Supressor of hairless, SUH, transcriptional, Notch, monoclonal, clone 7A11, sample

验证数据展示

  • Western blot of RBPJ antibody. Wild type (lane 1) or RBPJ KO (lane 2) DG75 cell lysate was probed with RBPJ antibody (top row) or a GAPDH antibody (bottom row) to show equal loading.

    Western blot of RBPJ antibody. Wild type (lane 1) or RBPJ KO (lane 2) DG75 cell lysate was probed with RBPJ antibody (top row) or a GAPDH antibody (bottom row) to show equal loading.

产品信息

Tested Applications WB

Applications Validated by Active Motif: WB: 2 - 4 ug/ml dilution
Tested Reactivity Human
Host / Isotype Rat / IgG2b
Class Monoclonal
Type Antibody
Immunogen This antibody was raised against full-length recombinant human RBPJ protein.
Full Name RBPJ antibody (mAb) (Clone 7A11)
Synonyms CBF-1, cbf1, RBPJk, RBPJ1, Supressor of hairless, SUH, transcriptional, Notch, monoclonal, clone 7A11, sample
Molecular weight 55 kDa
GenBank accession numberNP_005340
RRIDAB_2793496
Purification Method Protein G Chromatography
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

RBPJ (Recombination Signal Binding Protein For Immunoglobulin Kappa J Region) is a transcriptional regulator that plays a central role in Notch signaling, a signaling pathway involved in cell-cell communication that regulates a broad spectrum of cell-fate determinations. Acts as a transcriptional repressor when it is not associated with Notch proteins. When associated with some NICD product of Notch proteins (Notch intracellular domain), it acts as a transcriptional activator that activates transcription of Notch target genes. Probably represses or activates transcription via the recruitment of chromatin remodeling complexes containing histone deacetylase or histone acetylase proteins, respectively. Specifically binds to the immunoglobulin kappa-type J segment recombination signal sequence. Binds specifically to methylated DNA.