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验证数据展示

  • WB analysis of NIH/3T3 using 16484-1-AP

    WB analysis of NIH/3T3 using 16484-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16484-1-AP (RBM28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IP experiment of NIH/3T3 using 16484-1-AP

    IP experiment of NIH/3T3 using 16484-1-AP

    IP result of anti-RBM28 (IP:16484-1-AP, 4ug; Detection:16484-1-AP 1:1000) with NIH/3T3 cells lysate 1120 ug.

  • IF Staining of NIH/3T3 using 16484-1-AP

    IF Staining of NIH/3T3 using 16484-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 16484-1-AP (RBM28 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • WB analysis of U-251 using 16484-1-AP

    WB analysis of U-251 using 16484-1-AP

    WB result of RBM28 antibody (16484-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM28 transfected U-251 cells.

  • Featured Product
  • KD/KO验证

RBM28 Polyclonal antibody

RBM28 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 16484-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (1)

别名

RNA-binding protein 28, RNA-binding motif protein 28, RNA binding protein 28, RNA binding motif protein 28

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inNIH/3T3 cells, U-251 cells
Positive IP detected inNIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16484-1-AP targets RBM28 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human, mouse, rat
文献引用反应性human
免疫原 RBM28 fusion protein Ag9606 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 RNA binding motif protein 28
别名 RNA-binding protein 28, RNA-binding motif protein 28, RNA binding protein 28, RNA binding motif protein 28
计算分子量 756 aa, 86 kDa
观测分子量 100 kDa
GenBank蛋白编号BC013889
基因名称 RBM28
Gene ID (NCBI) 55131
RRIDAB_2238116
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NW13
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for RBM28 antibody 16484-1-APDownload protocol
IF protocol for RBM28 antibody 16484-1-APDownload protocol
IP protocol for RBM28 antibody 16484-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

J Biol Chem

RNA binding protein RBM28 can translocate from the nucleolus to the nucleoplasm to inhibit the transcriptional activity of p53.

Authors - Xin Lin
View Article
humanWB

Cancer Lett

RNA-binding motif protein 28 enhances angiogenesis by improving STAT3 translation in hepatocellular carcinoma

Authors - Hexu Han
View Article