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RB1 Polyclonal antibody

RB1 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)


WB, IP, ELISA and More (3)



Cat No : 10048-2-Ig

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OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1


Positive WB detected inA431 cells, Calyculin A treated Jurkat cells, MCF-7 cells
Positive IP detected inA431 cells


Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.


10048-2-Ig targets RB1 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IP, ELISA Application Description
Cited ApplicationsWB, IF, IHC, CoIP
Tested Reactivity human
Cited Reactivityhuman, zebrafish
Immunogen RB1 fusion protein Ag0006 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name retinoblastoma 1
Synonyms OSRC, p105 Rb, pp110, pRb, RB, RB1, retinoblastoma 1
Calculated Molecular Weight 110 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC040540
Gene Symbol RB1
Gene ID (NCBI) 5925
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.


RB1, also named as pp110, pRb and p105 Rb, belongs to the retinoblastoma protein (RB) family. It is a key regulator of entry into cell division that acts as a tumor suppressor. RB1 acts as a transcription repressor of E2F1 target genes. The underphosphorylated, active form of RB1 interacts with E2F1 and represses its transcription activity, leading to cell cycle arrest. It is directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. It recruits and targets histone methyltransferases SUV39H1, SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. RB1 controls histone H4 'Lys-20' trimethylation and inhibits the intrinsic kinase activity of TAF1. It mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity. This antibody is a rabbit polyclonal antibody raised against human RB1 fusion protein.


Product Specific Protocols
WB protocol for RB1 antibody 10048-2-IgDownload protocol
IP protocol for RB1 antibody 10048-2-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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