验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293T cells, A549 cells, A431 cells, U2OS cells, rat testis tissue, mouse testis tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
86839-3-RR targets RAB34 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag26696 Product name: Recombinant human RAB34 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 126-251 aa of BC016841 Sequence: QAIIIVFNLNDVASLEHTKQWLADALKENDPSSVLLFLTPAQYALMEKDALQVAQEMKAEYWAVSSLTGENVREFFFRVAALTFEANVLAELEKSGARRIGDVVRINSDDSNLYLTASKKKPTCCP 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | RAB34, member RAS oncogene family |
| 别名 | RAB39, RAH, Ras related protein Rab 34, Ras related protein Rab 39, Ras related protein Rah |
| 计算分子量 | 251 aa, 28 kDa |
| 观测分子量 | 29 kDa |
| GenBank蛋白编号 | BC016841 |
| 基因名称 | RAB34 |
| Gene ID (NCBI) | 83871 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9BZG1 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
RAB34, a Rab protein family member, is involved in repositioning of lysosomes, activation of micropinocytosis, and protein transport. RAB34 is aberrantly expressed in various cancers and exhibits oncogenic properties. Recently, Rab34 is identified as a ciliary protein. Rab34 is required for internal assembly of cilia but not for cilia built on the cell surface.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for RAB34 antibody 86839-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
