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验证数据展示

  • WB analysis of HeLa using 12349-1-AP

    WB analysis of HeLa using 12349-1-AP

    WB result of QTRT1 antibody (12349-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-QTRT1 transfected HeLa cells.

  • Featured Product
  • KD/KO验证

QTRT1 Polyclonal antibody

QTRT1 Polyclonal Antibody for WB, ELISA
Cat No. 12349-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

EC:2.4.2.64, FP3235, Guanine insertion enzyme, Queuine tRNA-ribosyltransferase catalytic subunit 1, TGT

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12349-1-AP targets QTRT1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 QTRT1 fusion protein Ag3010 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 queuine tRNA-ribosyltransferase 1
别名 EC:2.4.2.64, FP3235, Guanine insertion enzyme, Queuine tRNA-ribosyltransferase catalytic subunit 1, TGT
计算分子量 403 aa, 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC015350
基因名称 QTRT1
Gene ID (NCBI) 81890
RRIDAB_2877847
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9BXR0
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

QTRT1 (Queuine tRNA-ribosyltransferase catalytic subunit 1), also known as tRNA-guanine transglycosylase (TGT), is a key enzymeinvolved in the post-transcriptional modification of tRNAs. In humans, the QTRT1 and QTRT2 heterodimer complex exhibits transglycosylase activity, and replaces the guanine base of the first letter of the tRNA anticodon with q, thus forming Q on the tRNA (PMID: 30149595, 25884661). High QTRT1 expression is a biomarkerfor poor prognosis in LUAD patients, and QTRT1 mayfunction as a latent tumor biomarker in the prognosticprediction for LUAD (PMID: 33490170).

实验方案

Product Specific Protocols
WB protocol for QTRT1 antibody 12349-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols