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Purvalanol A

CAS号

212844-53-6

分子式

C19H25ClN6O

主要靶点

CDK|Apoptosis|Autophagy

仅限科研使用

Cat No : CM03400

Print datasheet

Synonyms

Inhibitor|NG 60|NG60|NG-60|Purvalanol A|inhibit|Cyclin dependent kinase|Apoptosis|CDK2/CyclinE|CDK4/CyclinD1|CDK2/CyclinA|CDK|Cdc2/CyclinB|Autophagy

验证数据展示

  • CAS No.: 212844-53-6

    Purvalanol A
    CAS#: 212844-53-6

产品信息

CAS号 212844-53-6
分子式 C19H25ClN6O
主要靶点 CDK|Apoptosis|Autophagy
主要通路 凋亡|细胞周期|自噬
分子量 388.89
纯度 98.91%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 Inhibitor|NG 60|NG60|NG-60|Purvalanol A|inhibit|Cyclin dependent kinase|Apoptosis|CDK2/CyclinE|CDK4/CyclinD1|CDK2/CyclinA|CDK|Cdc2/CyclinB|Autophagy

靶点活性

CDK2-CyclinE:35 nM|CDK4-CyclinD1:850 nM|CDK2-CyclinA:70 nM|CDC2-cyclinB:4 nM

体内活性

在MCF-7细胞中,Purvalanol A使细胞活性损失50%,但MDA-MB-231细胞对Purvalanol A的敏感性相对较低(细胞活性减少32 %)。在MCF-7和MDA-MB-231细胞中,Purvalanol A可促使线粒体介导的细胞凋亡。Purvalanol A可使MCF-7和MDA-MB-231细胞系的细胞活性降低,该作用具有剂量依赖性。通过抑制细胞周期进程和c-Src信号通路,Purvalanol A可有效防止c-Src介导转化,且对一些c-Src上调的人类癌细胞贴壁不依赖性生长有明显的抑制效果。Purvalanol A可显著抑制HT29和SW480人结肠癌细胞的贴壁依赖性生长。

溶解度

Ethanol:19.5 mg/mL (50 mM);DMSO:＜1 mg/mL (insoluble or slightly soluble)

细胞实验

Cells are seeded at 10000 density in 96-well plates and treated with various concentrations of Purvalanol A (0-100 μM) for 24 h. Cells are exposed to 10 μL of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltet- razolium bromide dye (5 mg/mL) and are incubated at 37℃ for 4 h. In order to solubilize the formazan crystals 100 μL DMSO is added. Absorbance is determined at 570 nm spectrophotometrically.(Only for Reference)

参考文献

1.Gray NS, et al. Science. 1998, 281(5376), 533-538.
2.Obakan P, et al. Mol Biol Rep. 2014, 41(1), 145-154.
3.Hikita T, et al. Genes Cells. 2010, 15(10), 1051-1062.

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
This equation is commonly abbreviated as: C1V1 = C2V2

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