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Puromycin dihydrochloride

CAS号

58-58-2

分子式

C22H31Cl2N7O5

主要靶点

Antibiotic|DNA/RNA Synthesis|Antibacterial|ribosome

仅限科研使用

Cat No : CM00466

Print datasheet

Synonyms

DNASynthesis|DNA Synthesis|DNA synthesis|inhibit|CL 13900|CL 13900 Dihydrochloride|CL13900|CL-13900|CL13900 dihydrochloride|CL13900 Dihydrochloride|CL-13900 Dihydrochloride|Bacterial|Antibiotic|60S ribosome|Inhibitor|Puromycin|Puromycin Dihydrochloride|Puromycin dihydrochloride|Puromycin 2HCl|RNA Synthesis|RNASynthesis|嘌呤霉素二盐酸盐|嘌呤霉素二盐酸盐水合物

验证数据展示

  • CAS No.: 58-58-2

    Puromycin dihydrochloride
    CAS#: 58-58-2

产品信息

CAS号 58-58-2
分子式 C22H31Cl2N7O5
主要靶点 Antibiotic|DNA/RNA Synthesis|Antibacterial|ribosome
主要通路 微生物学|微生物学|DNA 损伤和修复|微生物学|细胞周期
分子量 544.44
纯度 99.86%， 此纯度可做参考，具体纯度与批次有关系，可咨询客服
储存条件 store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.
别名 DNASynthesis|DNA Synthesis|DNA synthesis|inhibit|CL 13900|CL 13900 Dihydrochloride|CL13900|CL-13900|CL13900 dihydrochloride|CL13900 Dihydrochloride|CL-13900 Dihydrochloride|Bacterial|Antibiotic|60S ribosome|Inhibitor|Puromycin|Puromycin Dihydrochloride|Puromycin dihydrochloride|Puromycin 2HCl|RNA Synthesis|RNASynthesis|嘌呤霉素二盐酸盐|嘌呤霉素二盐酸盐水合物

体内活性

Puromycin能够用于从非培养细胞中选择重组细胞。在许多体系中Puromycin被用作研究蛋白质合成的工具。

体外活性

方法：人肝癌细胞 HepG2 和原代大鼠肝细胞 PRH 用 Puromycin dihydrochloride (0-300 μmol/L) 处理 72 h，使用 MTT 方法检测细胞生长抑制情况。 结果：Puromycin dihydrochloride 剂量依赖性地抑制 HepG2 和 PRH 细胞生长，IC50 分别为 1600 μmol/L 和 2000 nmol/L。[1] 方法：人乳腺癌细胞 MDA-MB-231 和 MDA-MB-436 用 shRNAs-Puro 慢病毒转染细胞，24 h 后，将转染的细胞在含有 Puromycin dihydrochloride (1 μg/ml) 的新培养基中培养约 12 天，筛选转染成功的细胞。 结果：Puromycin dihydrochloride 筛选出了慢病毒成功转染的细胞。[2] 方法：用慢病毒转染的细胞在补充有 Puromycin dihydrochloride (2 μg/mL) 的培养基中培养。 结果：Puromycin dihydrochloride 筛选并维持慢病毒成功转染的细胞。[3]

溶解度

DMSO:60 mg/mL (110.2 mM);H2O:92 mg/mL (168.98 mM);Ethanol:< 1 mg/mL (insoluble or slightly soluble)

参考文献

1.Chan C, et al. Incompatibility of chemical protein synthesis inhibitors with accurate measurement of extended protein degradation rates. Pharmacol Res Perspect. 2017 Oct;5(5):e00359.
2.Han W, et al. Roles of eIF3m in the tumorigenesis of triple negative breast cancer. Cancer Cell Int. 2020 Apr 29;20:141.
3.Wang YN, et al. Targeting the cholesterol-RORα/γ axis inhibits colorectal cancer progression through degrading c-myc. Oncogene. 2022 Dec;41(49):5266-5278.
4.Han W, Zhang C, Shi C, et al. Roles of eIF3m in the tumorigenesis of triple negative breast cancer[J]. Cancer Cell International. 2020, 20(1): 1-16.
5.Gao C, Wang H, Wang T, et al. Platelet CLEC‐2 regulates neuroinflammation and restores blood brain barrier integrity in a mouse model of traumatic brain injury[J]. Journal of Neurochemistry. 2020: e14983.

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