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验证数据展示

  • WB analysis using 18266-1-AP (same clone as 18266-1-PBS)

    WB analysis using 18266-1-AP (same clone as 18266-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18266-1-AP (Pinin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18266-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18266-1-AP (same clone as 18266-1-PBS)

    IF Staining of HeLa using 18266-1-AP (same clone as 18266-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Pinin antibody (18266-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18266-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18266-1-AP (same clone as 18266-1-PBS)

    IF Staining of HeLa using 18266-1-AP (same clone as 18266-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Pinin antibody (18266-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 18266-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 18266-1-AP (same clone as 18266-1-PBS)

    IP experiment of HeLa using 18266-1-AP (same clone as 18266-1-PBS)

    IP result of anti-Pinin (IP:18266-1-AP, 4ug; Detection:18266-1-AP 1:300) with HeLa cells lysate 520ug. This data was developed using the same antibody clone with 18266-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

Pinin Polyclonal antibody, PBS Only

Pinin Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 18266-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

SR-like protein, PNN, DRS, Domain-rich serine protein, Desmosome-associated protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

18266-1-PBS targets Pinin in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原 Pinin fusion protein Ag6643 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pinin, desmosome associated protein
别名 SR-like protein, PNN, DRS, Domain-rich serine protein, Desmosome-associated protein
计算分子量 82 kDa
观测分子量 82 kDa, 140 kDa
GenBank蛋白编号BC062602
基因名称 Pinin
Gene ID (NCBI) 5411
RRIDAB_10642138
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H307
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PNN, also named as DRS or 140 kDa nuclear and cell adhesion-related phosphoprotein, is a 717 amino acid protein, which belongs to the pinin family. PNN localizes in the plasma membrane and is expressed in placenta, lung, liver, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon, heart, epidermis, esophagus, brain and smooth and skeletal muscle. PNN is expressed strongly in melanoma metastasis lesions and advanced primary tumors. PNN as a transcriptional activator binds to the E-box 1 core sequence of the E-cadherin promoter gene. PNN is involved in the establishment and maintenance of epithelia cell-cell adhesion. PNN is a potential tumor suppressor for renal cell carcinoma. The calculated molecular weight of PNN is 82 kDa (native form), but the post-modified protein is 140 kDa (phospho form of isoform 1).