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验证数据展示

  • WB analysis using 28961-1-AP (same clone as 28961-1-PBS)

    WB analysis using 28961-1-AP (same clone as 28961-1-PBS)

    Non-treated HT-29 and etoposide treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28961-1-AP (Phospho-P53(Ser15) antibody) at dilution of 1:2000 incubated at 4℃ overnight. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28961-1-PBS in a different storage buffer formulation.

  • IF Staining of HT-29 using 28961-1-AP (same clone as 28961-1-PBS)

    IF Staining of HT-29 using 28961-1-AP (same clone as 28961-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed etoposide treated HT-29 cells using Phospho-P53 (Ser15) antibody (28961-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28961-1-PBS in a different storage buffer formulation.

  • WB analysis using 28961-1-AP (same clone as 28961-1-PBS)

    WB analysis using 28961-1-AP (same clone as 28961-1-PBS)

    Non-treated HSC-T6 cells and UV treated HSC-T6 cells were subjected to SDS PAGE followed by western blot with 28961-1-AP (Phospho-P53 (Ser15) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28961-1-PBS in a different storage buffer formulation.

Phospho-P53 (Ser15) Polyclonal antibody, PBS Only

Phospho-P53 (Ser15) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28961-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IF/ICC, Indirect ELISA

别名

p P53, p P53 (Ser15), p P53 Ser15, P53 (Ser15), Phospho P53

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy P53 Ready-To-Use IHC Kit. P53 primary antibody included.

产品信息

28961-1-PBS targets Phospho-P53 (Ser15) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, rat
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 tumor protein p53
别名 p P53, p P53 (Ser15), p P53 Ser15, P53 (Ser15), Phospho P53
计算分子量 44 kDa
观测分子量53 kDa
GenBank蛋白编号BC003596
基因名称 P53
Gene ID (NCBI) 7157
RRIDAB_2881236
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP04637
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis . DNA damage induces phosphorylation of p53 at Ser15 and Ser20 and leads to a reduced interaction between p53 and its negative regulator, the oncoprotein MDM2. p53 can be phosphorylated by ATM, ATR, and DNA-PK at Ser15 and Ser37. Phosphorylation impairs the ability of MDM2 to bind p53, promoting both the accumulation and activation of p53 in response to DNA damage.

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