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验证数据展示

  • WB analysis using 80093-1-RR (same clone as 80093-1-PBS)

    WB analysis using 80093-1-RR (same clone as 80093-1-PBS)

    Non-treated Ramos and Jurkat and Staurosporine treated Ramos and Jurkat cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (66240-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

  • WB analysis using 80093-1-RR (same clone as 80093-1-PBS)

    WB analysis using 80093-1-RR (same clone as 80093-1-PBS)

    Non-treated HeLa cells and Calyculin A treated HeLa cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Tubulin (66240-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

  • WB analysis using 80093-1-RR (same clone as 80093-1-PBS)

    WB analysis using 80093-1-RR (same clone as 80093-1-PBS)

    Non-treated NIH/3T3 cells and Calyculin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 80093-1-RR (Phospho-MST1 (Thr183)/MST2 (Thr180) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Beta Actin (66009-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 80093-1-RR (same clone as 80093-1-PBS)

    FC experiment of HeLa using 80093-1-RR (same clone as 80093-1-PBS)

    1X10^6 Calyculin A treated HeLa cells were intracellularly stained with 0.25 ug Anti-Human Phospho-MST1 (Thr183)/MST2 (Thr180) (80093-1-RR, Clone:1P6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.25 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with 90% MeOH. This data was developed using the same antibody clone with 80093-1-PBS in a different storage buffer formulation.

Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant antibody, PBS Only

Phospho-MST1 (Thr183)/MST2 (Thr180) Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 80093-1-PBS

产品说明书

CloneNo. 1P6

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

MST1 (Thr183)/MST2 (Thr180), p MST1 (Thr183)/MST2 (Thr180), Phospho MST1 (Thr183), Phospho MST1 (Thr183)/MST2 (Thr180), Phospho MST2 (Thr180)

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy STK4 Ready-To-Use IHC Kit. STK4 primary antibody included.

产品信息

80093-1-PBS targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 serine/threonine kinase 4
别名 MST1 (Thr183)/MST2 (Thr180), p MST1 (Thr183)/MST2 (Thr180), Phospho MST1 (Thr183), Phospho MST1 (Thr183)/MST2 (Thr180), Phospho MST2 (Thr180)
计算分子量 56 kDa
观测分子量 59 kDa
GenBank蛋白编号BC005231
基因名称 MST1
Gene ID (NCBI) 6789
RRIDAB_2918864
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ13043
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations.