验证数据展示
经过测试的应用
| Positive WB detected in | Serum-free treated NIH/3T3 cells, Insulin treated NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
29103-1-AP targets Phospho-MARCKS (Ser159/163) in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | myristoylated alanine-rich protein kinase C substrate |
| 别名 | MARCKS (Ser159/163), p MARCKS, p MARCKS (Ser159/163), p MARCKS Ser159/163, Phospho MARCKS |
| 计算分子量 | 32 kDa |
| 观测分子量 | 80 kDa |
| GenBank蛋白编号 | BC089040 |
| 基因名称 | MARCKS |
| Gene ID (NCBI) | 4082 |
| RRID | AB_3085342 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P29966 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The Myristoylated Alanine Rich C-Kinase Substrate (MARCKS) is a ubiquitous, highly conserved protein among vertebrates, which is essential for postnatal survival, and has been widely studied for its functions in the brain and nervous system. Being highly expressed in nervous tissue, particularly during early development but persisting in the adult, it plays numerous roles related to brain growth, neuronal migration, neurite outgrowth, neurotransmitter release, and synaptic plasticity. Protein kinase C (PKC) phosphorylates MARCKS, which converts MARCKS from a membrane-bound protein to a cytoplasmic protein. The phosphorylation site of MARCKS protein is called the effector domain (ED). Its structure is highly conserved. It can be combined with cell membrane, PKC, calcium/calmodulin-dependent kineses (CaMK) and F-actin. Studies have shown that increased membrane-bound, non-phosphorylated MARCKS might be conducive to the stabilization of synaptic morphology. Phosphorylated MARCKS protein (P-MARCKS) can regulate the stability of actin network and alter the synaptic structure. (PMID: 30655546, PMID: 30155805)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Phospho-MARCKS (Ser159/163) antibody 29103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |