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验证数据展示

  • WB analysis using 29574-1-AP

    WB analysis using 29574-1-AP

    Non-treated and UV treated HeLa cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

  • WB analysis using 29574-1-AP

    WB analysis using 29574-1-AP

    Non-treated and UV treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 29574-1-AP (Phospho-INPPL1 (Tyr986/987) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with INPPL1 antibody (26678-1-AP) subsequently.

Phospho-INPPL1 (Tyr986/987) Polyclonal antibody

Phospho-INPPL1 (Tyr986/987) Polyclonal Antibody for WB, ELISA
Cat No. 29574-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse

应用

WB, ELISA

别名

INPPL 1, INPPL1, Phospho-INPPL1 (Tyr986/987), Protein 51C, SHIP 2, SHIP2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inUV treated HeLa cells, UV treated NIH/3T3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy INPPL1 Ready-To-Use IHC Kit. INPPL1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29574-1-AP targets Phospho-INPPL1 (Tyr986/987) in WB, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 inositol polyphosphate phosphatase-like 1
别名 INPPL 1, INPPL1, Phospho-INPPL1 (Tyr986/987), Protein 51C, SHIP 2, SHIP2
计算分子量 1258 aa, 139 kDa
观测分子量150 kDa
GenBank蛋白编号BC140853
基因名称 INPPL1
Gene ID (NCBI) 3636
RRIDAB_3085347
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15357
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Inositol polyphosphate phosphatase-like protein 1(INPPL1) belongs to the inositol and phosphatidylinositol 5-phosphatase family, also known as SH2 domain-containing inositol 5'-phosphatase 2(SHIP2), is ubiquitous in all organizations. SHIP2 is constitutively tyrosine-phosphorylated in chronic myeloid leukemia progenitor cells. In B cells, SHIP2 was most tyrosine phosphorylated after BCR and FC-γriib crosslinking and was associated with Shc, but not after BCR stimulation alone. It has also been shown that SHIP2 can be phosphorylated at Tyr-986 in COS-7 cells and Chinese hamster ovary cells overexpressing the insulin receptor. (PMID: 11349134)

实验方案

Product Specific Protocols
WB protocol for Phospho-INPPL1 (Tyr986/987) antibody 29574-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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