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Conjugate
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验证数据展示

  • WB analysis using 28853-1-AP (same clone as 28853-1-PBS)

    WB analysis using 28853-1-AP (same clone as 28853-1-PBS)

    Non-treated HT-29 cells, phosphatase inhibitor and λ phosphatase treated HT-29 cells were subjected to SDS PAGE followed by western blot with 28853-1-AP (Phospho-Beta Catenin (Ser675) antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control. This data was developed using the same antibody clone with 28853-1-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 28853-1-AP (same clone as 28853-1-PBS)

    IF Staining of MCF-7 using 28853-1-AP (same clone as 28853-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed λ phosphatase treated MCF-7 cells using Phospho-Beta Catenin (Ser675) antibody (28853-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 28853-1-PBS in a different storage buffer formulation.

Phospho-Beta Catenin (Ser675) Polyclonal antibody, PBS Only

Phospho-Beta Catenin (Ser675) Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 28853-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy Beta Catenin Ready-To-Use IHC Kit. Beta Catenin primary antibody included.

产品信息

28853-1-PBS targets Phospho-Beta Catenin (Ser675) in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 catenin (cadherin-associated protein), beta 1, 88kDa
别名
计算分子量 781 aa, 86 kDa
观测分子量90-100 kDa
GenBank蛋白编号BC058926
基因名称 Beta Catenin
Gene ID (NCBI) 1499
ENSEMBL Gene IDENSG00000168036
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP35222
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Beta Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. Beta catenin is a core component of the cadherin protein complex, whose stabilization is essential for the activation of Wnt/β-catenin signaling. Phosphorylation of Ser675 in the C-terminus of at beta catenin promotesmits nuclear accumulation and transcriptional activity, resulting in increased activation of the Wnt/β-catenin signaling pathway(PMID: 25980439). In addition, Ser675 phosphorylation promotes beta catenin interaction with the transcriptional coactivator CREB-binding protein in the nucleus(PMID: 16476742).

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