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验证数据展示

  • WB analysis of HeLa using 10810-1-AP (same clone as 10810-1-PBS)

    WB analysis of HeLa using 10810-1-AP (same clone as 10810-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

    IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PUF60 antibody included
  • IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

    IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PUF60 antibody included
  • Featured Product
  • KD/KO验证

PUF60 Polyclonal antibody, PBS Only

PUF60 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10810-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PUF60 Ready-To-Use IHC Kit. PUF60 primary antibody included.

产品信息

10810-1-PBS targets PUF60 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag1053

Product name: Recombinant human PUF60 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 243-542 aa of BC008875

Sequence: DPTTGKHKGYGFIEYEKAQSSQDAVSSMNLFDLGGQYLRVGKAVTPPMPLLTPATPGGLPPAAAVAAAAATAKITAQEAVAGAAVLGTLGTPGLVSPALTLAQPLGTLPQAVMAAQAPGVITGVTPARPPIPVTIPSVGVVNPILASPPTLGLLEPKKEKEEEELFPESERPEMLSEQEHMSISGSSARHMVMQKLLRKQESTVMVLRNMVDPKDIDDDLEGEVTEECGKFGAVNRVIIYQEKQGEEEDAEIIVKIFVEFSIASETHKAIQALNGRWFAGRKVVAEVYDQERFDNSDLSA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly-U binding splicing factor 60KDa
别名
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC008875
基因名称 PUF60
Gene ID (NCBI) 22827
RRIDAB_2173583
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHX1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.