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验证数据展示

  • WB analysis of HeLa using 10810-1-AP (same clone as 10810-1-PBS)

    WB analysis of HeLa using 10810-1-AP (same clone as 10810-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10810-1-AP (PUF60 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

    IHC staining of human colon cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PUF60 antibody included
  • IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

    IHC staining of human breast cancer using 10810-1-AP (same clone as 10810-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10810-1-AP (PUF60 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10810-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PUF60 antibody included
  • Featured Product
  • KD/KO验证

PUF60 Polyclonal antibody, PBS Only

PUF60 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 10810-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy PUF60 Ready-To-Use IHC Kit. PUF60 primary antibody included.

产品信息

10810-1-PBS targets PUF60 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 PUF60 fusion protein Ag1053 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 poly-U binding splicing factor 60KDa
别名
计算分子量 60 kDa
观测分子量 60 kDa
GenBank蛋白编号BC008875
基因名称 PUF60
Gene ID (NCBI) 22827
RRIDAB_2173583
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UHX1
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Poly-U-binding factor 60(PUF60) is a splicing factor homologous to and complementary in function to U2AF, which involves in pre-mRNA splicing, a stepwise process initiated by binding. Also PUF60 involves in other nuclear processes such as apoptosis and transcription regulation. It can interact with and repress FUBP1-induced transcriptional activation via the core-TFIIH basal transcription factor. However, it has no repression activity in xeroderma pigmentosum complementation group B (XPB) cells.