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验证数据展示

  • IHC staining of rat brain using 12557-1-AP (same clone as 12557-1-PBS)

    IHC staining of rat brain using 12557-1-AP (same clone as 12557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12557-1-PBS in a different storage buffer formulation.

  • WB analysis of Y79 using 12557-1-AP (same clone as 12557-1-PBS)

    WB analysis of Y79 using 12557-1-AP (same clone as 12557-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 12557-1-AP (PRMT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12557-1-PBS in a different storage buffer formulation.

PRMT8 Polyclonal antibody, PBS Only

PRMT8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12557-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

12557-1-PBS targets PRMT8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag3166

Product name: Recombinant human PRMT8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 95-394 aa of BC022458

Sequence: RTLTYRNSMYHNKHVFKDKVVLDVGSGTGILSMFAAKAGAKKVFGIECSSISDYSQKIIKANHLDNIITIFKGKVEEVELPVEKVDIIISEWMGYCLFYESMLNTVIFARDKWLKPGGLMFPDRAALYVVAIEDRQYKDFKIHWWENVYGFDMTCIRDVAMKEPLVDIVDPKQVVTNACLIKEVDIYTVKTEELSFTSAFCLQIQRNDYVHALVTYFNIEFTKCHKKMGFSTAPDAPYTHWKQTVFYLEDYLTVRRGEEIYGTISMKPNAKNVRDLDFTVDLDFKGQLCETSVSNDYKMR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein arginine methyltransferase 8
别名
计算分子量 394 aa, 45 kDa
观测分子量 43-50 kDa
GenBank蛋白编号BC022458
基因名称 PRMT8
Gene ID (NCBI) 56341
RRIDAB_2171964
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NR22
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRMT8 is a member of arginine methyl transferase (PRMT) family which acts as nuclear receptor coactivators. PRMT8 catalyzes the asymmetric arginine methylation and is involved in cellular differentiation. PRMT8 has several isoforms; the full-length isoform has a brain specific expression pattern and is localized to the plasma membrane; the truncated isoforms display nuclear localization and are expressed more widely. This antibody recognizes all of isoforms of PRMT8.

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