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验证数据展示

  • WB analysis using 83969-7-RR (same clone as 83969-7-PBS)

    WB analysis using 83969-7-RR (same clone as 83969-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83969-7-RR (PRMT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83969-7-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83969-7-RR (same clone as 83969-7-PBS)

    IF Staining of HeLa using 83969-7-RR (same clone as 83969-7-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRMT3 antibody (83969-7-RR, Clone: 241092E6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83969-7-PBS in a different storage buffer formulation.

  • FC experiment of A549 using 83969-7-RR (same clone as 83969-7-PBS)

    FC experiment of A549 using 83969-7-RR (same clone as 83969-7-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-7-RR, Clone:241092E6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 83969-7-PBS in a different storage buffer formulation.

PRMT3 Recombinant monoclonal antibody, PBS Only

PRMT3 Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 83969-7-PBS

产品说明书

CloneNo. 241092E6

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC (Intra), ELISA

别名

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83969-7-PBS targets PRMT3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag13466

Product name: Recombinant human PRMT3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 178-531 aa of BC037544

Sequence: RAREDLQKMKQFAQDFVMHTDVRTCSSSTSVIADLQEDEDGVYFSSYGHYGIHEEMLKDKIRTESYRDFIYQNPHIFKDKVVLDVGCGTGILSMFAAKAGAKKVLGVDQSEILYQAMDIIRLNKLEDTITLIKGKIEEVHLPVEKVDVIISEWMGYFLLFESMLDSVLYAKNKYLAKGGSVYPDICTISLVAVSDVNKHADRIAFWDDVYGFKMSCMKKAVIPEAVVEVLDPKTLISEPCGIKHIDCHTTSISDLEFSSDFTLKITRTSMCTAIAGYFDIYFEKNCHNRVVFSTGPQSTKTHWKQTVFLLEKPFSVKAGEALKGKVTVHKNKKDPRSLTVTLTLNNSTQTYGLQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 protein arginine methyltransferase 3
别名 Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319
计算分子量 531 aa, 60 kDa
观测分子量60-68 kDa
GenBank蛋白编号BC037544
基因名称 PRMT3
Gene ID (NCBI) 10196
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purfication
UNIPROT IDO60678
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.

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