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PRKRA Monoclonal antibody

PRKRA Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D4C3

Cat No : 68518-1-Ig

Print datasheet

Synonyms

DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX

验证数据展示

  • WB analysis using 68518-1-Ig

    WB analysis using 68518-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68518-1-Ig (PRKRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68518-1-Ig targets PRKRA in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications WB, ELISA
Tested Reactivity Human, mouse
Immunogen PRKRA fusion protein Ag29818 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name protein kinase, interferon-inducible double stranded RNA dependent activator
Synonyms DYT16, HSD 14, HSD14, PACT, PKR associated protein X, PKR associating protein X, PRKRA, RAX
Calculated Molecular Weight 34 kDa
Observed Molecular Weight 34 kDa
GenBank Accession NumberBC009470
Gene Symbol PRKRA
Gene ID (NCBI) 8575
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75569
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PRKRA (PKR-associated protein X) is also named as PACT, RAX, HSD-14 and HSD14. PRKRA was significantly overexpressed in PC tissues and promoted the proliferation, migration, and chemoresistance of PC cells (PMID: 37484321). PRKRA activates EIF2AK2/PKR in the absence of double-stranded RNA (dsRNA), leading to phosphorylation of EIF2S1/EFI2-alpha and inhibition of translation and induction of apoptosis. PRKRA has been shown to interact with DICER1. DICER1 and PRKRA expression in normal colon was limited to mucosal epithelium and showed similar cytoplasmic expression (PMID: 37484321).

实验方案

Product Specific Protocols
WB protocol for PRKRA antibody 68518-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols