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验证数据展示

  • WB analysis using 28351-1-AP (same clone as 28351-1-PBS)

    WB analysis using 28351-1-AP (same clone as 28351-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 28351-1-AP (PRKAR2B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 28351-1-AP (same clone as 28351-1-PBS)

    IF Staining of Neuro-2a using 28351-1-AP (same clone as 28351-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

  • IF Staining of Neuro-2a using 28351-1-AP (same clone as 28351-1-PBS)

    IF Staining of Neuro-2a using 28351-1-AP (same clone as 28351-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Neuro-2a cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 28351-1-AP (same clone as 28351-1-PBS)

    IP experiment of mouse brain using 28351-1-AP (same clone as 28351-1-PBS)

    IP result of anti-PRKAR2B (IP:28351-1-AP, 4ug; Detection:28351-1-AP 1:1000) with mouse brain tissue lysate 1080 ug. This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

  • IF Staining of SH-SY5Y using 28351-1-AP (same clone as 28351-1-PBS)

    IF Staining of SH-SY5Y using 28351-1-AP (same clone as 28351-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using PRKAR2B antibody (28351-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

  • FC experiment of SH-SY5Y using 28351-1-AP (same clone as 28351-1-PBS)

    FC experiment of SH-SY5Y using 28351-1-AP (same clone as 28351-1-PBS)

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.4 ug Anti-Human PRKAR2B (28351-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach using 28351-1-AP (same clone as 28351-1-PBS)

    IHC staining of human stomach using 28351-1-AP (same clone as 28351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 28351-1-AP (same clone as 28351-1-PBS)

    IHC staining of human stomach cancer using 28351-1-AP (same clone as 28351-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 28351-1-AP (PRKAR2B antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28351-1-PBS in a different storage buffer formulation.

PRKAR2B Polyclonal antibody, PBS Only

PRKAR2B Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 28351-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

28351-1-PBS targets PRKAR2B in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag28787

Product name: Recombinant human PRKAR2B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-122 aa of BC075800

Sequence: MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGTARFCHEGRTWGDLGAAAGGGTPSKGVNFAEEPMQSDSEDGEEEEAAPADAGAFNAPVINRFTRRASVCAEAYNP

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase, cAMP-dependent, regulatory, type II, beta
别名 RII BETA, PRKAR2, cAMP-dependent protein kinase type II-beta regulatory subunit
计算分子量 418 aa, 46 kDa
观测分子量46-50 kDa
GenBank蛋白编号BC075800
基因名称 PRKAR2B
Gene ID (NCBI) 5577
RRIDAB_2918156
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP31323
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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