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验证数据展示

  • IHC staining of human small intestine using 21654-1-AP

    IHC staining of human small intestine using 21654-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21654-1-AP (PPYR1 antibody) at dilution of 1:200 (under 40x lens).

  • IHC staining of human small intestine using 21654-1-AP

    IHC staining of human small intestine using 21654-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 21654-1-AP (PPYR1 Antibody) at dilution of 1:50 (under 10x lens).

PPYR1 Polyclonal antibody

PPYR1 Polyclonal Antibody for IHC, ELISA
Cat No. 21654-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA

别名

Neuropeptide Y receptor type 4, PP1, PPYR1, Y4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21654-1-AP targets PPYR1 in IHC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16374

Product name: Recombinant human PPYR1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 317-375 aa of BC096238

Sequence: VNPFIYGFLNTNFKKEIKALVLTCQQSAPLEESEHLPLSTVHTEVSKGSLRLSGRSNPI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 pancreatic polypeptide receptor 1
别名 Neuropeptide Y receptor type 4, PP1, PPYR1, Y4
计算分子量 375 aa, 42 kDa
GenBank蛋白编号BC096238
基因名称 PPYR1
Gene ID (NCBI) 5540
RRIDAB_2878900
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50391
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PPYR1 (pancreatic polypeptide receptor 1), also known as NPY4R, is a member of the G-protein coupled receptor 1 family. PPYR1 is a receptor for the PP-fold peptides (pancreatic polypeptide, peptide YY, and neuropeptide Y) and pancreatic polypeptide is the preferred ligand (PMID: 12626767). It may be regulating central nervous system, cardiovascular and gastrointestinal function. It has been reported that PPYR1 gene is a key regulator of energy homeostasis (PMID: 12000791). PPYR1 gene copy number gain has been associated with lower body mass index (BMI) (PMID: 19229253).

实验方案

Product Specific Protocols
IHC protocol for PPYR1 antibody 21654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols