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验证数据展示

  • WB analysis using 13482-1-AP (same clone as 13482-1-PBS)

    WB analysis using 13482-1-AP (same clone as 13482-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13482-1-AP (PPP2CA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 13482-1-AP (same clone as 13482-1-PBS)

    IP experiment of Jurkat using 13482-1-AP (same clone as 13482-1-PBS)

    IP result of anti-PPP2CA (IP:13482-1-AP, 4ug; Detection:13482-1-AP 1:500) with Jurkat cells lysate 2400ug. This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 13482-1-AP (same clone as 13482-1-PBS)

    IHC staining of mouse liver using 13482-1-AP (same clone as 13482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPP2CA antibody included
  • IHC staining of mouse liver using 13482-1-AP (same clone as 13482-1-PBS)

    IHC staining of mouse liver using 13482-1-AP (same clone as 13482-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 13482-1-AP (PPP2CA antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PPP2CA antibody included
  • IF Staining of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    IF Staining of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP2CA antibody (13482-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • FC experiment of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    FC experiment of MCF-7 using 13482-1-AP (same clone as 13482-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.2 ug Anti-Human PPP2CA (13482-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 13482-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PPP2CA Polyclonal antibody, PBS Only

PPP2CA Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 13482-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

EC:3.1.3.16, PP2A alpha, PP2A-alpha, PP2Ac, PP2CA

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PPP2CA Ready-To-Use IHC Kit. PPP2CA primary antibody included.

产品信息

13482-1-PBS targets PPP2CA in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4294

Product name: Recombinant human PPP2CA protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-309 aa of BC031696

Sequence: MDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
别名 EC:3.1.3.16, PP2A alpha, PP2A-alpha, PP2Ac, PP2CA
计算分子量 309 aa, 36 kDa
观测分子量 36 kDa
GenBank蛋白编号BC031696
基因名称 PPP2CA
Gene ID (NCBI) 5515
RRIDAB_2169485
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP67775
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PPP2CA, also named as RP-C and PP2A-alpha, belongs to the PPP phosphatase family and PP-1 subfamily. PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. PP2A activity was modestly decreased in AML patients harboring C-KIT/WT.PP2A is a target for C-KIT/TKD and implicated the potential efficacy of therapies targeting PP2A in such AML in future.

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