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验证数据展示

  • IHC staining of mouse brain using 25295-1-AP

    IHC staining of mouse brain using 25295-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25295-1-AP (PPFIA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 25295-1-AP

    IHC staining of mouse brain using 25295-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25295-1-AP (PPFIA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PPFIA4 Polyclonal antibody

PPFIA4 Polyclonal Antibody for IHC, ELISA
Cat No. 25295-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IHC, ELISA

别名

KIAA0897, Liprin alpha 4, PPFIA4

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25295-1-AP targets PPFIA4 in IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18130

Product name: Recombinant human PPFIA4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-78 aa of BC132925

Sequence: MGSAADVRFSLGTTTHAPPGVHRRYSALREESAKDWETSPLPGMLAPAAGPAFDSDPEISDVDEDEPGGLVGSADVVS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 4
别名 KIAA0897, Liprin alpha 4, PPFIA4
计算分子量 701 aa, 78 kDa
GenBank蛋白编号BC132925
基因名称 PPFIA4
Gene ID (NCBI) 8497
RRIDAB_3085783
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75335
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tyrosine phosphatase receptor type F polypeptide interacting protein alpha 4 (PPFIA4), also named as Liprin alpha 4, belongs to the liprin family and Liprin-alpha subfamily. PPFIA4 has been reported as a new potential therapeutic target for refractory pancreatic cancer, small cell lung cancer, and clear cell renal cell cancer (PMID: 35382861). As a glycolysis-related oncogene, PPFIA4 is also a potential biomarker of colon cancer (PMID: 34094943).

实验方案

Product Specific Protocols
IHC protocol for PPFIA4 antibody 25295-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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