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POLL Polyclonal antibody

POLL Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat No : 29920-1-AP

Print datasheet

Synonyms

BETA N, DNA polymerase beta 2, DNA polymerase kappa, DNA polymerase lambda, FLJ46002, Pol beta2, POL KAPPA, Pol Lambda, POLL

验证数据展示

  • WB analysis using 29920-1-AP

    WB analysis using 29920-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29920-1-AP (POLL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 29920-1-AP

    WB analysis using 29920-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29920-1-AP (POLL antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inL02 cells, HEK-293 cells, HepG2 cells, HEK-293T cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29920-1-AP targets POLL in WB, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, ELISA
Tested Reactivity Human
Immunogen POLL fusion protein Ag31995 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name polymerase (DNA directed), lambda
Synonyms BETA N, DNA polymerase beta 2, DNA polymerase kappa, DNA polymerase lambda, FLJ46002, Pol beta2, POL KAPPA, Pol Lambda, POLL
Calculated Molecular Weight 63 kDa
Observed Molecular Weight63-70 kDa
GenBank Accession NumberBC068529
Gene Symbol POLL
Gene ID (NCBI) 27343
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UGP5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLL (DNA polymerase lambda) functions in DNA repair (PMID: 11457865, PMID: 19806195, PMID: 20693240, PMID: 30250067). POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, indicating that POLL participates in short- and long-patch base excision repair. POLL is involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PMID: 11457865, PMID: 19806195).

实验方案

Product Specific Protocols
WB protocol for POLL antibody 29920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols