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验证数据展示

  • WB analysis using 29920-1-AP

    WB analysis using 29920-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29920-1-AP (POLL antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • WB analysis using 29920-1-AP

    WB analysis using 29920-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29920-1-AP (POLL antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

POLL Polyclonal antibody

POLL Polyclonal Antibody for WB, ELISA
Cat No. 29920-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

BETA N, DNA polymerase beta 2, DNA polymerase kappa, DNA polymerase lambda, FLJ46002, Pol beta2, POL KAPPA, Pol Lambda, POLL

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inL02 cells, HEK-293 cells, HepG2 cells, HEK-293T cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:5000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

29920-1-AP targets POLL in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原

CatNo: Ag31995

Product name: Recombinant human POLL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 389-575 aa of BC068529

Sequence: REEATEIEQTVQKAAQAFNSGLLCVACGSYRRGKATCGDVDVLITHPDGRSHRGIFSRLLDSLRQEGFLTDDLVSQEENGQQQKYLGVCRLPGPGRRHRRLDIIVVPYSEFACALLYFTGSAHFNRSMRALAKTKGMSLSEHALSTAVVRNTHGCKVGPGRVLPTPTEKDVFRLLGLPYREPAERDW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), lambda
别名 BETA N, DNA polymerase beta 2, DNA polymerase kappa, DNA polymerase lambda, FLJ46002, Pol beta2, POL KAPPA, Pol Lambda, POLL
计算分子量 63 kDa
观测分子量63-70 kDa
GenBank蛋白编号BC068529
基因名称 POLL
Gene ID (NCBI) 27343
RRIDAB_3086189
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UGP5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

POLL (DNA polymerase lambda) functions in DNA repair (PMID: 11457865, PMID: 19806195, PMID: 20693240, PMID: 30250067). POLL has 5-prime-deoxyribose-5-phosphate lyase activity and strand-displacement synthesis activity on gapped DNA substrates, indicating that POLL participates in short- and long-patch base excision repair. POLL is involved in base excision repair (BER) responsible for repair of lesions that give rise to abasic (AP) sites in DNA (PMID: 11457865, PMID: 19806195).

实验方案

Product Specific Protocols
WB protocol for POLL antibody 29920-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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