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验证数据展示

  • WB analysis using 15646-1-AP (same clone as 15646-1-PBS)

    WB analysis using 15646-1-AP (same clone as 15646-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15646-1-AP (POLD1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

    IHC staining of human breast cancer using 15646-1-AP (same clone as 15646-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15646-1-AP (POLD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 15646-1-AP (same clone as 15646-1-PBS)

    IP experiment of HeLa using 15646-1-AP (same clone as 15646-1-PBS)

    IP result of anti-POLD1 (IP:15646-1-AP, 4ug; Detection:15646-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 15646-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

POLD1 Polyclonal antibody, PBS Only

POLD1 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15646-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

15646-1-PBS targets POLD1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag8178

Product name: Recombinant human POLD1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 858-1107 aa of BC008800

Sequence: EGAVAHAQDVISDLLCNRIDISQLVITKELTRAASDYAGKQAHVELAERMRKRDPGSAPSLGDRVPYVIISAAKGVAAYMKSEDPLFVLEHSLPIDTQYYLEQQLAKPLLRIFEPILGEGRAEAVLLRGDHTRCKTVLTGKVGGLLAFAKRRNCCIGCRTVLSHQGAVCEFCQPRESELYQKEVSHLNALEERFSRLWTQCQRCQGSLHEDVICTSRDCPIFYMRKKVRKDLEDQEQLLRRFGPPGPEAW

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 polymerase (DNA directed), delta 1, catalytic subunit 125kDa
别名
计算分子量 1107 aa, 124 kDa
观测分子量 124 kDa
GenBank蛋白编号BC008800
基因名称 POLD1
Gene ID (NCBI) 5424
RRIDAB_2252572
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP28340
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

POLD1, also known as DNA polymerase subunit delta p125, is 1107 amino acid protein, which belongs to the DNA polymerase type-B family. POLD1 colocalizes with PCNA and POLD3 at S phase replication sites (PubMed:11595739). As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. A previous study demonstrated that overexpression of POLD1 is associated with an increased risk of breast cancer. (PMID: 30344713 )

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