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验证数据展示

  • IF Staining of HeLa using CL488-83260-5

    IF Staining of HeLa using CL488-83260-5

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 PLK1-phospho antibody (CL488-83260-5, Clone: 240092B10 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • IF Staining of HepG2 using CL488-83260-5

    IF Staining of HepG2 using CL488-83260-5

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 PLK1-phospho antibody (CL488-83260-5, Clone: 240092B10 ) at dilution of 1:200, Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

CoraLite® Plus 488-conjugated PLK1-phospho Recombinant antibody

PLK1-phospho Recombinant Antibody for IF/ICC
Cat No. CL488-83260-5

产品说明书

CloneNo. 240092B10

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC

别名

PLK1, 240092B10, EC:2.7.11.21, PLK, PLK 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHepG2 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy PLK1 Ready-To-Use IHC Kit. PLK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-83260-5 targets PLK1-phospho in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 polo-like kinase 1 (Drosophila)
别名 PLK1, 240092B10, EC:2.7.11.21, PLK, PLK 1
计算分子量 68 kDa
GenBank蛋白编号NM_005030
基因名称 PLK1
Gene ID (NCBI) 5347
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP53350
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 PLK1-phospho antibody CL488-83260-5Download protocol
Standard Protocols
Click here to view our Standard Protocols