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验证数据展示

  • WB analysis using 18088-1-AP (same clone as 18088-1-PBS)

    WB analysis using 18088-1-AP (same clone as 18088-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18088-1-AP (PLA2G4E antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

    IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

    IHC staining of human skeletal muscle using 18088-1-AP (same clone as 18088-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 18088-1-AP (PLA2G4E antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 18088-1-AP (same clone as 18088-1-PBS)

    IF Staining of HeLa using 18088-1-AP (same clone as 18088-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PLA2G4E antibody (18088-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 18088-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PLA2G4E Polyclonal antibody, PBS Only

PLA2G4E Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 18088-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

phospholipase A2, group IVE, PLA2G4E Ens

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

18088-1-PBS targets PLA2G4E in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag12808

Product name: Recombinant human PLA2G4E protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 519-868 aa of BC101584

Sequence: KDDVSNQDFREWFEFSPYEVGLQKYGAFIPSELFGSEFFMGRLVKRIPESRICYMLGLWSSIFSLNLLDAWNLSHTSEEFFHRWTREKVQDIEDEPILPEIPKCDANILETTVVIPGSWLSNSFREILTHRSFVSEFHNFLSGLQLHTNYLQNGQFSRWKDTVLDGFPNQLTESANHLCLLDTAFFVNSSYPPLLRPERKADLIIHLNYCAGSQTKPLKQTCEYCTVQNIPFPKYELPDENENLKECYLMENPQEPDAPIVTFFPLINDTFRKYKAPGVERSPEELEQGQVDIYGPKTPYATKELTYTEATFDKLVKLSEYNILNNKDTLLQALRLAVEKKKRLKGQCPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phospholipase A2, group IVE
别名 phospholipase A2, group IVE, PLA2G4E Ens
计算分子量 868 aa, 99 kDa
观测分子量 99 kDa, 57 kDa
GenBank蛋白编号BC101584
基因名称 PLA2G4E
Gene ID (NCBI) 123745
RRIDAB_10859777
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ3MJ16
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PLA2G4E (Phospholipase A2 group IVE), also known as cPLA2-epsilon. Calcium-dependent N-acyltransferase is involved in the biosynthesis of N-acyl ethanolamines (NAEs) in the brain (PMID:29447909). Transfers the sn-1 fatty acyl chain of phosphatidylcholine (fatty acyl donor) to the amine group of phosphatidylethanolamine (fatty acyl acceptor) to generate N-acyl phosphatidylethanolamine (NAPE). Similarly can use plasmenylethanolamine as a fatty acyl acceptor to form N-acyl plasmenylethanolamine (N-Acyl-PlsEt). Both NAPE and N-Acyl-PlsEt can serve as precursors of bioactive NAEs like N-arachidonoyl phosphatidylethanolamine also called anandamide (PMID:29447909; 30517655).

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