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验证数据展示

  • WB analysis using 29208-1-AP (same clone as 29208-1-PBS)

    WB analysis using 29208-1-AP (same clone as 29208-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29208-1-AP (PKC Gamma antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

    IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29208-1-AP (PKC Gamma antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

    IHC staining of mouse cerebellum using 29208-1-AP (same clone as 29208-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 29208-1-AP (PKC Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29208-1-PBS in a different storage buffer formulation.

PKC Gamma Polyclonal antibody, PBS Only

PKC Gamma Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29208-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

29208-1-PBS targets PKC Gamma in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag25085

Product name: Recombinant human PRKCG protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 600-641 aa of BC047876

Sequence: SGPDGEPTIRAHGFFRWIDWERLERLEIPPPFRPRPCGRSGE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 protein kinase C, gamma
别名
计算分子量 78 kDa
观测分子量78 kDa
GenBank蛋白编号BC047876
基因名称 PKC Gamma
Gene ID (NCBI) 5582
RRIDAB_2918249
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP05129
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC gamma is a neuron-specific member of the classical PKCs and is activated and translocated to subcellular regions as a result of various stimuli, including diacylglycerol synthesis, increased intracellular Ca(2+) and phorbol esters. Defects in this protein have been associated with spinocerebellar ataxia type 14 (SCA14), an autosomal dominant neurodegenerative disease.

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