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验证数据展示

  • WB analysis using 68484-1-Ig

    WB analysis using 68484-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68484-1-Ig (PISD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 68484-1-Ig

    WB analysis using 68484-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68484-1-Ig (PISD antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

PISD Monoclonal antibody

PISD Monoclonal Antibody for WB, ELISA
Cat No. 68484-1-Ig

产品说明书

CloneNo. 3B6C1

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

3B6C1, EC:4.1.1.65, Phosphatidylserine decarboxylase alpha chain, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase proenzyme, mitochondrial

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, LNCaP cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68484-1-Ig targets PISD in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag9499

Product name: Recombinant human PISD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-375 aa of BC001482

Sequence: MMCQSEARQGPELRAAKWLHFPQLALRRRLGQLSCMSRPALKLRSWPLTVLYYLLPFGALRPLSRVGWRPVSRVALYKSVPTRLLSRAWGRLNQVELPHWLRRPVYSLYIWTFGVNMKEAAVEDLHHYRNLSEFFRRKLKPQARPVCGLHSVISPSDGRILNFGQVKNCEVEQVKGVTYSLESFLGPRMCTEDLPFPPAASCDSFKNQLVTREGNELYHCVIYLAPGDYHCFHSPTDWTVSHRRHFPGSLMSVNPGMARWIKELFCHNERVVLTGDWKHGFFSLTAVGATNVGSIRIYFDRDLHTNSPRHSKGSYNDFSFVTHTNREGVPMRKGEHLGEFNLGSTIVLIFEAPKDFNFQLKTGQKIRFGEALGSL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 phosphatidylserine decarboxylase
别名 3B6C1, EC:4.1.1.65, Phosphatidylserine decarboxylase alpha chain, Phosphatidylserine decarboxylase beta chain, Phosphatidylserine decarboxylase proenzyme, mitochondrial
计算分子量375aa,43 kDa; 408aa,46 kDa
观测分子量46 kDa
GenBank蛋白编号BC001482
基因名称 PISD
Gene ID (NCBI) 23761
RRIDAB_3085195
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9UG56
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

PISD(Phosphatidylserine decarboxylase proenzyme) belongs to the phosphatidylserine decarboxylase family.It contains a conserved LGST motif that functions as an autocatalytic cleavage site where the proenzyme is split into mature alpha and beta subunits.It has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for PISD antibody 68484-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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