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验证数据展示

  • WB analysis using 19427-1-AP (same clone as 19427-1-PBS)

    WB analysis using 19427-1-AP (same clone as 19427-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19427-1-AP (PIH1D1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

    IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

    IHC staining of human lung using 19427-1-AP (same clone as 19427-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung using 19427-1-AP (PIH1D1 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

    IF Staining of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

    Immunofluorescent analysis of Hela cells, using PIH1D1 antibody 19427-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

    IP experiment of HeLa using 19427-1-AP (same clone as 19427-1-PBS)

    IP result of anti-PIH1D1 (IP:19427-1-AP, 4ug; Detection:19427-1-AP 1:1000) with HeLa cells lysate 1200ug. This data was developed using the same antibody clone with 19427-1-PBS in a different storage buffer formulation.

PIH1D1 Polyclonal antibody, PBS Only

PIH1D1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 19427-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

19427-1-PBS targets PIH1D1 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag13759

Product name: Recombinant human PIH1D1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-290 aa of BC001108

Sequence: MANPKLLGLELSEAEAIGADSARFEELLLQASKELQQAQTTRPESTQIQPQPGFCIKTNSSEGKVFINICHSPSIPPPADVTEEELLQMLEEDQAGFRIPMSLGEPHAELDAKGQGCTAYDVAVNSDFYRRMQNSDFLRELVITIAREGLEDKYNLQLNPEWRMMKNRPFMGSISQQNIRSEQRPRIQELGDLYTPAPGRAESGPEKPHLNLWLEAPDLLLAEIDLPKLDGALGLSLEIGENRLVMGGPQQLYHLDAYIPLQINSHESKAAFHRKRKQLMVAMPLLLVPS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PIH1 domain containing 1
别名
计算分子量 290 aa, 32 kDa
观测分子量 39 kDa
GenBank蛋白编号BC001108
基因名称 PIH1D1
Gene ID (NCBI) 55011
RRIDAB_10640423
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NWS0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PIH1D1 (PIH1 domain-containing protein 1; also known as PIH1 or NOP17), is a ortholog of the yeast nucleolar protein NOP17. PIH1D1 is a conserved subunit of the R2TP complex participating in the assembly of snoRNP and RNA polymerase II complexes. PIH1D1 may also function as a novel modulator of apoptosis pathway.

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