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验证数据展示

  • WB analysis of HeLa using 20421-1-AP

    WB analysis of HeLa using 20421-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20421-1-AP (PIGM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20421-1-AP

    IHC staining of human testis using 20421-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20421-1-AP (PIGM antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 20421-1-AP

    IHC staining of human testis using 20421-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20421-1-AP (PIGM antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 20421-1-AP

    IF Staining of HeLa using 20421-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20421-1-AP (PIGM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

PIGM Polyclonal antibody

PIGM Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 20421-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, ELISA

别名

PIG-M, PIG M, Phosphatidylinositol-glycan biosynthesis class M protein, GPI-MT-I, GPI mannosyltransferase 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20421-1-AP targets PIGM in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag14245

Product name: Recombinant human PIGM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 231-299 aa of BC019865

Sequence: VAVAGLTFFALSFGFYYEYGWEFLEHTYFYHLTRRDIRHNFSPYFYMLYLTAESKWSFSLGIAAFLPQL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphatidylinositol glycan anchor biosynthesis, class M
别名 PIG-M, PIG M, Phosphatidylinositol-glycan biosynthesis class M protein, GPI-MT-I, GPI mannosyltransferase 1
计算分子量 423 aa, 49 kDa
观测分子量 50 kDa
GenBank蛋白编号BC019865
基因名称 PIGM
Gene ID (NCBI) 93183
RRIDAB_10734320
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H3S5
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIGM antibody 20421-1-APDownload protocol
IHC protocol for PIGM antibody 20421-1-APDownload protocol
IF protocol for PIGM antibody 20421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols