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PIGM Polyclonal antibody

PIGM Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 20421-1-AP

Print datasheet

Synonyms

GPI mannosyltransferase 1, GPI mannosyltransferase I, GPI MT I, PIG M, PIGM

验证数据展示

  • WB analysis of HeLa using 20421-1-AP

    WB analysis of HeLa using 20421-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 20421-1-AP (PIGM antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 20421-1-AP

    IHC staining of human testis using 20421-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20421-1-AP (PIGM antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 20421-1-AP

    IHC staining of human testis using 20421-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 20421-1-AP (PIGM antibody) at dilution of 1:100 (under 40x lens).

  • IF Staining of HeLa using 20421-1-AP

    IF Staining of HeLa using 20421-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 20421-1-AP (PIGM antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IHC detected inhuman testis tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20421-1-AP targets PIGM in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity human, mouse, rat
Immunogen PIGM fusion protein Ag14245 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatidylinositol glycan anchor biosynthesis, class M
Synonyms GPI mannosyltransferase 1, GPI mannosyltransferase I, GPI MT I, PIG M, PIGM
Calculated Molecular Weight 423 aa, 49 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC019865
Gene Symbol PIGM
Gene ID (NCBI) 93183
RRIDAB_10734320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H3S5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PIGM antibody 20421-1-APDownload protocol
IHC protocol for PIGM antibody 20421-1-APDownload protocol
IF protocol for PIGM antibody 20421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols