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验证数据展示

  • WB analysis using 25244-1-AP (same clone as 25244-1-PBS)

    WB analysis using 25244-1-AP (same clone as 25244-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 25244-1-AP (PHLPP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    WB analysis of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    WB result of PHLPP2 antibody (25244-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PHLPP2 transfected HeLa cells. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    IP experiment of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    IP result of anti-PHLPP2 (IP:25244-1-AP, 4ug; Detection:25244-1-AP 1:500) with HeLa cells lysate 1880 ug. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

    IHC staining of human colon cancer using 25244-1-AP (same clone as 25244-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25244-1-AP (PHLPP2 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    IF Staining of HeLa using 25244-1-AP (same clone as 25244-1-PBS)

    Immunofluorescent analysis of HeLa cells using 25244-1-AP (PHLPP2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 25244-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

PHLPP2 Polyclonal antibody, PBS Only

PHLPP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 25244-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

PHLPPL, EC:3.1.3.16, KIAA0931, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

25244-1-PBS targets PHLPP2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag19000

Product name: Recombinant human PHLPPL protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 575-781 aa of BC129927

Sequence: LDLQHNALTRLPDTLFSKALNLRYLNASANSLESLPSACTGEESLSMLQLLYLTNNLLTDQCIPVLVGHLHLRILHLANNQLQTFPASKLNKLEQLEELNLSGNKLKTIPTTIANCKRLHTLVAHSNNISIFPEILQLPQIQFVDLSCNDLTEILIPEALPATLQDLDLTGNTNLVLEHKTLDIFSHITTLKIDQKPLPTTDSTVTS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PH domain and leucine rich repeat protein phosphatase-like
别名 PHLPPL, EC:3.1.3.16, KIAA0931, PH domain leucine-rich repeat-containing protein phosphatase 2, PH domain leucine-rich repeat-containing protein phosphatase-like
计算分子量 1323 aa, 147 kDa
观测分子量 147 kDa
GenBank蛋白编号BC129927
基因名称 PHLPPL
Gene ID (NCBI) 23035
RRIDAB_2879985
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6ZVD8
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

PHLPP2 also named as PHLPPL is a 1323 amino acid protein, which contains 22 LRR repeats, 1 PH domain and 1 PP2C-like domain. Localized to both the nucleus and the cytoplasm, PHLPPL uses manganese as a cofactor and mediates the dephosphorylation of Akt1, thereby playing a role in cell survival and apoptotic regulation. Multiple isoforms of PHLPPL exist due to alternative splicing events.

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