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Host
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Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
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Compatible Species
0
Conjugate
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验证数据展示

  • WB analysis using 29516-1-AP (same clone as 29516-1-PBS)

    WB analysis using 29516-1-AP (same clone as 29516-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 29516-1-AP (PHF8 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHF8 antibody included
  • IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    IHC staining of human colon cancer using 29516-1-AP (same clone as 29516-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29516-1-AP (PHF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 29516-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with PHF8 antibody included

PHF8 Polyclonal antibody, PBS Only

PHF8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 29516-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy PHF8 Ready-To-Use IHC Kit. PHF8 primary antibody included.

产品信息

29516-1-PBS targets PHF8 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag30701

Product name: Recombinant human PHF8 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 786-948 aa of NM_001184896

Sequence: GQDRSSGSSSSGLGTVSNSPASQRTPGKRPIKRPAYWRTESEEEEENASLDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSN

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 PHD finger protein 8
别名
计算分子量 118 kDa
观测分子量125-135 kDa
GenBank蛋白编号NM_001184896
基因名称 PHF8
Gene ID (NCBI) 23133
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UPP1
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Plant homeodomain finger protein 8 (PHF8) is a JmjC domain-containing protein. PHF8 is localized in the nucleolus and may regulate rRNA transcription. As a member of the KDM7 subfamily of enzymes, PHF8 is involved in demethylation of lysine residues in histones such as H3K27me2/1, H3K9me2/1 and H4K20me1. PHF8 has been reported to participate in cancer development and metastasis of various types of tumors(PMID: 33111613). PHF8 has 5 isoforms produced by alternative splicing(Uniprot).

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