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PHACTR1 Polyclonal antibody

PHACTR1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 23446-1-AP

Print datasheet

Synonyms

KIAA1733, PHACTR1, RPEL, RPEL1

验证数据展示

  • WB analysis using 23446-1-AP

    WB analysis using 23446-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23446-1-AP (PHACTR1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 23446-1-AP

    IHC staining of mouse brain using 23446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23446-1-AP (PHACTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 23446-1-AP

    IHC staining of mouse brain using 23446-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 23446-1-AP (PHACTR1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

23446-1-AP targets PHACTR1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications IHC, WB, ELISA
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen PHACTR1 fusion protein Ag20125 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name phosphatase and actin regulator 1
Synonyms KIAA1733, PHACTR1, RPEL, RPEL1
Calculated Molecular Weight 580 aa, 66 kDa
Observed Molecular Weight 66-70 kDa
GenBank Accession NumberBC101526
Gene Symbol PHACTR1
Gene ID (NCBI) 221692
RRIDAB_2879281
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ9C0D0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for PHACTR1 antibody 23446-1-APDownload protocol
IHC protocol for PHACTR1 antibody 23446-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Pediatr

Analysis and comparisons of gene expression changes in patient- derived neurons from ROHHAD, CCHS, and PWS

Authors - A Kaitlyn Victor
View Article