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PGK1 Polyclonal antibody

PGK1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 17811-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (1)

应用

WB, IF/ICC, IP, ELISA and More (3)

Cell migration-inducing gene 10 protein, EC:2.7.2.3, OK/SW-cl.110, PGKA, PRP 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, HEK-293 cells, HeLa cells, PC-12 cells, PC-3 cells, NIH/3T3 cells, mouse liver tissue, rat liver tissue
Positive IP detected inSW 1990 cells, HeLa cells
Positive IF/ICC detected inA431 cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PGK1 Ready-To-Use IHC Kit. PGK1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

17811-1-AP targets PGK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig
免疫原 PGK1 fusion protein Ag12119 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 phosphoglycerate kinase 1
别名 Cell migration-inducing gene 10 protein, EC:2.7.2.3, OK/SW-cl.110, PGKA, PRP 2
计算分子量 417 aa, 45 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC103752
基因名称 PGK1
Gene ID (NCBI) 5230
RRIDAB_2161218
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00558
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The PGK1 gene encodes phosphoglycerate kinase-1, also known as ATP:3-phosphoglycerate 1-phosphotransferase (EC 2.7.2.3), which catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate during glycolysis, generating one molecule of ATP. It Belongs to the phosphoglycerate kinase family and defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D). PGK1 has 2 isoforms with the molecular mass of 45 kDa and 41 kDa.

实验方案

Product Specific Protocols
WB protocol for PGK1 antibody 17811-1-APDownload protocol
IF protocol for PGK1 antibody 17811-1-APDownload protocol
IP protocol for PGK1 antibody 17811-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Cancer Cell

Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.

Authors - Ling Li
mouseIF

Nat Neurosci

A glycolytic shift in Schwann cells supports injured axons.

Authors - Elisabetta Babetto
humanWB,IP,IF

Mol Cell

The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.

Authors - Youming Zhu
humanWB

Nat Commun

Low chorionic villous succinate accumulation associates with recurrent spontaneous abortion risk.

Authors - Xiao-Hui Wang
humanWB

Nat Commun

Fasting inhibits aerobic glycolysis and proliferation in colorectal cancer via the Fdft1-mediated AKT/mTOR/HIF1α pathway suppression.

Authors - Mei-Lin Weng
humanWB,IHC,IF,CoIP

Exp Hematol Oncol

Downregulation of pro-surfactant protein B contributes to the recurrence of early-stage non-small cell lung cancer by activating PGK1-mediated Akt signaling

Authors - Hao Luo
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