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验证数据展示

  • WB analysis using 85743-1-RR

    WB analysis using 85743-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85743-1-RR (PELO antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 85743-1-RR

    IF Staining of HeLa using 85743-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PELO antibody (85743-1-RR, Clone: 243137F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 85743-1-RR

    Affinity and Kinetic Characterization of 85743-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85743-1-RR against Human PELO were performed. The affinity constant is below 1 pM.

PELO Recombinant antibody

PELO Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85743-1-RR

产品说明书

CloneNo. 243137F4

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, MDA-MB-231 cells, HEK-293 cells
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

85743-1-RR targets PELO in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 PELO fusion protein Ag0873 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 pelota homolog (Drosophila)
别名 CGI 17, CGI-17, hPelota, PRO1770, Protein Dom34 homolog
计算分子量 43 kDa
观测分子量 43-45 kDa
GenBank蛋白编号BC005889
基因名称 PELO
Gene ID (NCBI) 53918
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9BRX2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The Pelo gene was originally identified in a mutagenesis screen for spermatogenesis-specific genes of Drosophila melanogaster. The PELO is required for the meiotic division during the G2/M transition, and functions in recognizing stalled ribosomes and triggering endonucleolytic cleavage of the mRNA, a mechanism to release non-functional ribosomes and degrade damaged mRNAs [PMID:12556505]. It may participate in the machinery of protein synthesis or in the regulation of mRNA translation [PMID:9584085].

实验方案

Product Specific Protocols
WB protocol for PELO antibody 85743-1-RRDownload protocol
IF protocol for PELO antibody 85743-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols